Nanostructural materials increase mineralization in bone cells and affect gene expression through miRNA regulation

We report that several nanomaterials induced enhanced mineralization (increased numbers and larger areas of mineral nests) in MC3T3‐E1 bone cells, with the highest response being induced by silver nanoparticles (AgNPs). We demonstrate that AgNPs altered microRNA expression resulting in specific gene...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine 2011-11, Vol.15 (11), p.2297-2306
Main Authors: Mahmood, Meena, Li, Zhiguang, Casciano, Daniel, Khodakovskaya, Mariya V., Chen, Tao, Karmakar, Alokita, Dervishi, Enkeleda, Xu, Yang, Mustafa, Thikra, Watanabe, Fumiya, Fejleh, Ashley, Whitlow, Morgan, Al‐Adami, Mustafa, Ghosh, Anindya, Biris, Alexandru S.
Format: Article
Language:English
Subjects:
3T3 Cells
Alkaline Phosphatase - metabolism
Animals
Bone and Bones - cytology
bone cells
Bone growth
Bone morphogenetic proteins
Bone Morphogenetic Proteins - metabolism
Calcification
Calcification, Physiologic - drug effects
Calcification, Physiologic - genetics
Carbon
Cell activation
Cell Line
Cells
Electron microscopes
Gene Expression
Genetic engineering
Metal Nanoparticles
Mice
MicroRNAs
MicroRNAs - metabolism
Mineralization
miRNA
miRNA regulation
Nanomaterials
Nanoparticles
Nanostructures
Nanotechnology
Original
Osteoblasts - metabolism
Osteogenesis
Proteins
Scanning electron microscopy
Silver
Spectrum analysis
Transcription activation
Transcription factors
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Summary:We report that several nanomaterials induced enhanced mineralization (increased numbers and larger areas of mineral nests) in MC3T3‐E1 bone cells, with the highest response being induced by silver nanoparticles (AgNPs). We demonstrate that AgNPs altered microRNA expression resulting in specific gene expression associated with bone formation. We suggest that the identified essential transcriptional factors and bone morphogenetic proteins play an important role in activation of the process of mineralization in bone cells exposed to AgNPs.
ISSN:1582-1838
1582-4934
DOI:10.1111/j.1582-4934.2010.01234.x