Dominance of P/Q-type calcium channels in depolarization-induced presynaptic fm dye release in cultured hippocampal neurons

Highlights • We analyzed depolarization-induced synaptic FM dye release in hippocampal neurons. • We pharmacologically isolated the contribution of voltage-gated Ca2+ channels. • 85% of synapses utilize N- and P/Q-type channels, 15% only P/Q-type channels. • In both groups of synapses release kineti...

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Bibliographic Details
Published in:Neuroscience 2013-12, Vol.253, p.330-340
Main Authors: Nimmervoll, B, Flucher, B.E, Obermair, G.J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cadmium Chloride - pharmacology
Calcium - metabolism
Calcium Channel Blockers - pharmacology
calcium channel physiology
Calcium Channels, N-Type - metabolism
Cells, Cultured
Dose-Response Relationship, Drug
Embryo, Mammalian
Female
Fluorescent Dyes - pharmacokinetics
Fundamental and applied biological sciences. Psychology
Hippocampus - cytology
Mice
Mice, Inbred BALB C
N-type
Neurology
Neurons - cytology
Neurons - drug effects
Neurons - metabolism
neurotransmitter release
omega-Agatoxin IVA - pharmacology
omega-Conotoxin GVIA - pharmacology
P/Q-type
Potassium Chloride - pharmacology
Presynaptic Terminals - drug effects
Presynaptic Terminals - metabolism
Pyridinium Compounds - pharmacokinetics
Quaternary Ammonium Compounds - pharmacokinetics
synapse function
Vertebrates: nervous system and sense organs
voltage-gated Ca2+ channels
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Summary:Highlights • We analyzed depolarization-induced synaptic FM dye release in hippocampal neurons. • We pharmacologically isolated the contribution of voltage-gated Ca2+ channels. • 85% of synapses utilize N- and P/Q-type channels, 15% only P/Q-type channels. • In both groups of synapses release kinetics are determined by P/Q-type channels. • We propose a more direct coupling of P/Q-type channels to synaptic release.
ISSN:0306-4522
1873-7544
DOI:10.1016/j.neuroscience.2013.08.052