Association of GSTM1 and GSTT1 polymorphism with lipid peroxidation in benign prostate hyperplasia and prostate cancer: a pilot study

Association of glutathione S-transferase (GST) M1 and T1 deletions with benign prostate hyperplasia (BPH) and prostate cancer is well reported. These enzymes metabolize numerous toxins thus protecting from oxidative injury. Oxidative stress has been associated with development of BPH and prostate ca...

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Bibliographic Details
Published in:Disease markers 2011, Vol.30 (4), p.163-169
Main Authors: Kumar, Vivek, Yadav, Chandra Shekhar, Datta, Sudip Kumar, Singh, Satyender, Ahmed, Rafat S, Goel, Sanjay, Gupta, Sanjay, Mustafa, Md, Grover, Rajesh Kumar, Banerjee, Basu Dev
Format: Article
Language:English
Subjects:
Aged
Biomarkers - blood
Cross-Sectional Studies
Genotype
Glutathione Transferase - genetics
Humans
Lipid Peroxidation - genetics
Male
Malondialdehyde - blood
Middle Aged
Other
Oxidative Stress - genetics
Oxidative Stress - physiology
Pilot Projects
Polymerase Chain Reaction
Polymorphism, Genetic
Prostatic Hyperplasia - genetics
Prostatic Neoplasms - genetics
Sequence Deletion
Smoking
Tobacco, Smokeless
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Summary:Association of glutathione S-transferase (GST) M1 and T1 deletions with benign prostate hyperplasia (BPH) and prostate cancer is well reported. These enzymes metabolize numerous toxins thus protecting from oxidative injury. Oxidative stress has been associated with development of BPH and prostate cancer. The present study was designed to analyze role of GST deletions in development of oxidative stress in these subjects. GSTs are responsible for metabolism of toxins present in tobacco therefore effect of tobacco usage in study groups was also studied. Three groups of subjects: BPH (53 patients), prostate cancer (57 patients) and controls (46 subjects) were recruited [corrected]. Genotyping was done using a multiplex polymerase chain reaction (PCR) method. Malondialdehyde (MDA) levels as marker of oxidative stress were estimated by measuring thiobarbituric acid reactive substance (TBARS) in plasma. Based on genotyping, subjects were categorized into: GSTM1+/GSTT1+, GSTM1-/GSTT1+, GSTM1+/GSTT1- and GSTM1-/GSTT1-. Significantly higher plasma MDA levels were noticed in GSTM1-/GSTT1- as compared to GSTM1+/GSTT1+ in all study groups. Double deletion (GSTM1-/GSTT1-) is associated with higher oxidative stress which might play a role in the pathogenesis of BPH and prostate cancer. However, other markers of oxidative stress should be analyzed before any firm conclusion.
ISSN:0278-0240
1875-8630
DOI:10.3233/DMA-2011-0774