Streptacidiphilus hamsterleyensis sp. nov., isolated from a spruce forest soil

• Published in: Antonie van Leeuwenhoek 2013-12, Vol.104 (6), p.965-972
• Main Authors: Golinska, Patrycja, Kim, Byung-Yong, Dahm, Hanna, Goodfellow, Michael
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial Typing Techniques; Biomedical and Life Sciences; Cluster Analysis; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Forest soils; Life Sciences; Medical Microbiology; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Original Paper; Phylogenetics; Phylogeny; Plant Sciences; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Soil microorganisms; Soil Science & Conservation; Streptomycetaceae - classification; Streptomycetaceae - genetics; Streptomycetaceae - isolation & purification; Taxonomy; Trees
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-013-0015-1

Three acidophilic actinobacteria, isolates LSCA2, FGG8 and HSCA14 T , recovered from spruce litter were examined using a polyphasic approach. Chemotaxonomic and morphological properties of the isolates were found to be consistent with their classification in the genus Streptacidiphilus . The isolates were shown to have identical 16S rRNA gene sequences and were most closely related to Streptacidiphilus neutrinimicus DSM 41755 T (99.9 % similarity). However, DNA:DNA relatedness between isolate HSCA14 T and the type strain of S. neutrinimicus was found to be low at 44.0 (±14.1) %. A combination of phenotypic features, including degradative and nutritional characteristics were shown to distinguish the isolates from their nearest phylogenetic neighbours. Data from this study show that the isolates form a novel species in the genus for which the name S. hamsterleyensis sp. nov. is proposed. The type strain is HSCA 14 T (=DSM 45900 T  = KACC 17456 T  = NCIMB 14865 T ).