Competitive Inhibition of Colchicine Binding to Tubulin by Microtubule-Associated Proteins

Microtubule-associated proteins (MAPs) promote tubulin polymerization, whereas colchicine inhibits this process. In this paper, MAPs have been shown to inhibit colchicine binding to tubulin in a competitive manner. Attempts were made to identify which of the MAPs fraction(s) was responsible: both ta...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1979-01, Vol.76 (1), p.86-90
Main Authors: Nunez, Jacques, Fellous, Arlette, Francon, Jacques, Lennon, Ana Maria
Format: Article
Language:English
Subjects:
Animals
Binding Sites
Binding, Competitive
Biochemistry
Brain
Chromatography
Colchicine - metabolism
Gels
Glycoproteins - metabolism
Kinetics
Magnesium - pharmacology
Microtubule associated proteins
Microtubules
Microtubules - metabolism
Molecular Weight
Molecules
Nerve Tissue Proteins - metabolism
Polymerization
Protein Binding
Rats
Tau proteins
Tubulin - metabolism
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Description
Summary:Microtubule-associated proteins (MAPs) promote tubulin polymerization, whereas colchicine inhibits this process. In this paper, MAPs have been shown to inhibit colchicine binding to tubulin in a competitive manner. Attempts were made to identify which of the MAPs fraction(s) was responsible: both tau protein (a thermostable molecule with a molecular weight of ≈ 70,000) and a high molecular weight fraction (HMW) were able to compete with colchicine. In contrast, Mg2+, which also induces microtubule assembly in vitro, had no effect on colchicine binding to tubulin.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.76.1.86