Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrela...

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Bibliographic Details
Published in:Biomedical optics express 2013-11, Vol.4 (11), p.2347-2361
Main Authors: Nadort, Annemarie, Woolthuis, Rutger G, van Leeuwen, Ton G, Faber, Dirk J
Format: Article
Language:English
Subjects:
Speckle Imaging and Diagnostics
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Summary:We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources.
ISSN:2156-7085
2156-7085
DOI:10.1364/boe.4.002347