Methylene tetrahydrofolate reductase and angiotensin converting enzyme gene polymorphisms related to overweight/obesity among Saudi subjects from Qassim Region

• Published in: Disease markers 2009-01, Vol.27 (2), p.97-102
• Main Authors: Settin, Ahmad A, Algasham, Abdullah, Dowaidar, Moataz, Ismail, Hisham
• Format: Article
• Language: English
• Subjects: Other
• ISSN: 0278-0240; 1875-8630
• DOI: 10.3233/DMA-2009-0660

BACKGROUNDThis work was planned to check for the association of polymorphisms related to methylenetetrahydrofolate reductase (MTHFR) and angiotensin converting enzyme (ACE) genes with overweight/obesity among Saudi subjects from Qassim region. METHODSThis work included 130 subjects having overweight or obesity and 111 normal controls. Their age mean +/- SD was 27 +/- 9.8 and 24 +/- 8.8 years respectively. Their DNA was analyzed for polymorphisms of MTHFR; 677C/T and 1298 A/C and ACE; I/D genes using real-time PCR. RESULTSGenotype and allele frequencies of studied polymorphisms in cases of overweight/obesity showed no significant statistical difference compared to that of controls. However, on analysis of body mass index (BMI), cases showed slightly higher but statistically nonsignificant mean +/- SD values among those carrying the mutant MTHFR 677 T allele (CT + TT vs. CC, 30.7 +/- 4.5 vs. 29.9 +/- 4.9), 1298 C allele (AC + CC vs. AA, 29.9 +/- 4.1 vs. 29.7 +/- 5.5) and ACE D allele (ID + DD vs. II, 30.0 +/- 5.1 vs. 29.1 +/- 2.8). In addition controls having the DD and ID genotypes showed higher statistically significant values of BMI than those of the II genotype (22.0 +/- 1.9, 21.7 +/- 2.6 and 19.5 +/- 2.3 respectively, p < 0.05). CONCLUSIONThere is no solid association of polymorphisms related to MTHFR and ACE genes with non-complicated overweight or obesity among Saudi subjects from Qassim Region.