Mature Adult Dystrophic Mouse Muscle Environment Does Not Impede Efficient Engrafted Satellite Cell Regeneration and Self‐Renewal

Changes that occur in the skeletal muscle environment with the progress of muscular dystrophies may affect stem cell function and result in impaired muscle regeneration. It has previously been suggested that the success of stem cell transplantation could therefore be dependent both on the properties...

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Bibliographic Details
Published in:Stem cells (Dayton, Ohio) Ohio), 2009-10, Vol.27 (10), p.2478-2487
Main Authors: Boldrin, Luisa, Zammit, Peter Steven, Muntoni, Francesco, Morgan, Jennifer Elizabeth
Format: Article
Language:English
Subjects:
Age Factors
Aged
Animals
Cell Communication - physiology
Cell Survival - physiology
Disease Models, Animal
Graft Survival - physiology
Mice
Mice, Inbred mdx
Mice, Transgenic
Muscle Fibers, Skeletal - metabolism
Muscle Proteins - metabolism
Muscle, Skeletal - metabolism
Muscle, Skeletal - physiopathology
Muscular dystrophy
Muscular Dystrophy, Animal - surgery
Regeneration - physiology
Satellite cell
Satellite Cells, Skeletal Muscle - cytology
Satellite Cells, Skeletal Muscle - physiology
Satellite Cells, Skeletal Muscle - transplantation
Self‐renewal
Skeletal muscle regeneration
Stem cell
Stem Cell Transplantation - methods
Treatment Outcome
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Summary:Changes that occur in the skeletal muscle environment with the progress of muscular dystrophies may affect stem cell function and result in impaired muscle regeneration. It has previously been suggested that the success of stem cell transplantation could therefore be dependent both on the properties of the cell itself and on the host muscle environment. Here we engrafted young and mature adult mdx‐nude mice, which are the genetic homolog of Duchenne muscular dystrophy, with a small number of satellite cells freshly isolated from young, normal donor mice. We found that the donor satellite cells contributed to muscle regeneration and self‐renewal as efficiently within mature adult, as in young, dystrophic host muscle. Donor‐derived satellite cells also contributed to robust regeneration after further injury, showing that they were functional despite the more advanced dystrophic muscle environment. These findings provide evidence that muscle tissue in a later stage of dystrophy may be effectively treated by stem cells. STEM CELLS 2009;27:2478–2487
ISSN:1066-5099
1549-4918
DOI:10.1002/stem.162