Young developmental age cardiac extracellular matrix promotes the expansion of neonatal cardiomyocytes in vitro

A major limitation to cardiac tissue engineering and regenerative medicine strategies is the lack of proliferation of postnatal cardiomyocytes. The extracellular matrix (ECM) is altered during heart development, and studies suggest that it plays an important role in regulating myocyte proliferation....

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Bibliographic Details
Published in:Acta biomaterialia 2014-01, Vol.10 (1), p.194-204
Main Authors: Williams, C., Quinn, K.P., Georgakoudi, I., Black, L.D.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Attachment
Biomedical materials
Cardiac tissue engineering
Cardiomyocyte
Cell Adhesion
Cell Proliferation
Design engineering
Electrochemical machining
Extracellular matrix
Extracellular Matrix - metabolism
Heart - growth & development
Heart Ventricles - cytology
In vitro testing
Markers
Myocardium - cytology
Myocytes, Cardiac - cytology
Proliferation
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Second Harmonic Generation imaging
Strategy
Tissue engineering
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Summary:A major limitation to cardiac tissue engineering and regenerative medicine strategies is the lack of proliferation of postnatal cardiomyocytes. The extracellular matrix (ECM) is altered during heart development, and studies suggest that it plays an important role in regulating myocyte proliferation. Here, the effects of fetal, neonatal and adult cardiac ECM on the expansion of neonatal rat ventricular cells in vitro are studied. At 24h, overall cell attachment was lowest on fetal ECM; however, ∼80% of the cells were cardiomyocytes, while many non-myocytes attached to older ECM and poly-l-lysine controls. After 5days, the cardiomyocyte population remained highest on fetal ECM, with a 4-fold increase in number. Significantly more cardiomyocytes stained positively for the mitotic marker phospho-histone H3 on fetal ECM compared with other substrates at 5days, suggesting that proliferation may be a major mechanism of cardiomyocyte expansion on young ECM. Further study of the beneficial properties of early developmental aged cardiac ECM could advance the design of novel biomaterials aimed at promoting cardiac regeneration.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2013.08.037