Whole‐body recruitment of glycocalyx volume during intravenous adenosine infusion

• Published in: Physiological reports 2013-10, Vol.1 (5), p.e00102-n/a
• Main Authors: Brands, Judith, Haare, Judith, Vink, Hans, VanTeeffelen, Jurgen W. G. E.
• Format: Article
• Language: English
• Subjects: Adenosine; Blood pressure; Cardiovascular disease; Cardiovascular system; Coronary vessels; Erythrocytes; glycocalyx; Hematocrit; indicator dilution; Intravenous administration; Laboratory animals; Microvasculature; Molecular weight; Original Research; Ostomy; Physiology; Recruitment; Surgery; systemic; Vasodilation
• ISSN: 2051-817X; 2051-817X
• DOI: 10.1002/phy2.102

Adenosine‐mediated recruitment of microvascular volume in heart and muscle has been suggested to include, in addition to vasodilation of resistance vessels, an increased accessibility of the endothelial glycocalyx for flowing plasma as a result of an impairment of its barrier properties. The aim of the current study was to investigate the effect of systemic intravenous administration of adenosine on the glycocalyx‐dependent exclusion of circulating blood at a whole‐body level. In anesthetized goats (N = 6), systemic blood‐excluded glycocalyx volume was measured by comparing the intravascular distribution volume of the suggested glycocalyx accessible tracer dextrans with a molecular weight of 40 kDa (Dex‐40) to that of circulating plasma, derived from the dilution of labeled red blood cells and large vessel hematocrit. Systemic glycocalyx volume was determined at baseline and during intravenous infusion of adenosine (157 ± 11.6 μg/kg min−1). Blood‐inaccessible glycocalyx volume decreased from 458.1 ± 95.5 to 18.1 ± 62.2 mL (P