A Transmissible Retrovirus Expressing Human Hypoxanthine Phosphoribosyltransferase (HPRT): Gene Transfer into Cells Obtained from Humans Deficient in HPRT

A cDNA corresponding to the human gene for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) has been ligated into murine retroviral vectors such that it is under the transcriptional control of viral long terminal repeats. Transfection of HPRT-cel...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1983-08, Vol.80 (15), p.4709-4713
Main Authors: Miller, A. Dusty, Jolly, Douglas J., Friedmann, Theodore, Verma, Inder M.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cell lines
Cloning, Molecular
Complementary DNA
DNA
DNA - metabolism
Genes
Genes, Viral
Genetic vectors
HeLa cells
Helper viruses
Helper Viruses - genetics
Humans
Hypoxanthine Phosphoribosyltransferase - deficiency
Hypoxanthine Phosphoribosyltransferase - genetics
Mice
Mice, Inbred BALB C
NIH 3T3 cells
Rats
Rats, Inbred F344
Retroviridae
Retroviridae - enzymology
Retroviridae - genetics
RNA
Simian virus 40 - genetics
Transfection
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cDNA corresponding to the human gene for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) has been ligated into murine retroviral vectors such that it is under the transcriptional control of viral long terminal repeats. Transfection of HPRT-cells followed by superinfection with various helper viruses has led to the rescue of chimeric virus capable of transmitting the HPRT+phenotype to HPRT-rodent or human cells. These genetically transformed cells contain authentic human HPRT at levels similar to normal HPRT+cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.15.4709