PreImplantation factor (PIF) detection in maternal circulation in early pregnancy correlates with live birth (bovine model)

Early identification of viable pregnancy is paramount for successful reproduction. Detection of specific signals from pre-implantation viable embryos in normal pregnancy circulation would indicate initiation of embryo-maternal interaction and create a continuum to accurately reflect embryo/fetal wel...

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Bibliographic Details
Published in:Reproductive biology and endocrinology 2013-11, Vol.11 (1), p.105-105, Article 105
Main Authors: Ramu, Sivakumar, Stamatkin, Christopher, Timms, Leo, Ruble, Marshall, Roussev, Roumen G, Barnea, Eytan R
Format: Article
Language:English
Subjects:
Animals
Biomarkers - blood
Biomarkers - chemistry
Cattle
Clinical trials
Embryo, Mammalian - metabolism
Embryonic Development
Embryos
Enzyme-Linked Immunosorbent Assay
Female
Live Birth
Pregnancy
Proprietary
Ultrasonic imaging
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Summary:Early identification of viable pregnancy is paramount for successful reproduction. Detection of specific signals from pre-implantation viable embryos in normal pregnancy circulation would indicate initiation of embryo-maternal interaction and create a continuum to accurately reflect embryo/fetal well-being post-implantation. Viable mammalian embryos secrete PreImplantation Factor (PIF), a biomarker which plays key, multi-targeted roles to promote implantation, trophoblast invasion and modulate maternal innate and adaptive immunity toward acceptance. Anti-PIF monoclonal antibody (mAb-based chemiluminescent ELISA) accurately detects PIF in singly cultured embryos media and its increased levels correlate with embryo development up to the blastocyst stage. Herein reported that PIF levels (ELISA) in early maternal serum correlate with pregnancy outcome. Artificially inseminated (AI) blind-coded Angus cattle (N = 21-23) serum samples (day 10,15 & 20 post-AI) with known calf birth were blindly tested, using both non-pregnant heifers (N = 30) and steer serum as negative controls. Assay properties and anti-PIF monoclonal antibody specificity were determined by examining linearity, spike and recovery experiments and testing the antibody against 234 different circulating proteins by microarray. Endogenous PIF was detected using
ISSN:1477-7827
1477-7827
DOI:10.1186/1477-7827-11-105