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Anti-inflammatory, cytotoxic and antioxidant effects of methanolic extracts of Drypetes sepiaria (Euphorbiaceae)
The aim of this study was to investigate in vitro antioxidant, anti-inflammatory and cytotoxic activities of the petroleum ether, ethyl acetate, methanol and aqueous extracts obtained from leaves of Drypetes sepiaria (Euphorbiaceae). Total phenolic and flavonoid contents of these crude extracts were...
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| Published in: | African journal of traditional, complementary, and alternative medicines complementary, and alternative medicines, 2013-01, Vol.10 (5), p.274-282 |
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| Language: | English |
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| container_end_page | 282 |
| container_issue | 5 |
| container_start_page | 274 |
| container_title | African journal of traditional, complementary, and alternative medicines |
| container_volume | 10 |
| creator | Gadamsetty, Ganesh Maru, Saurabh Tyagi, Abhishek Chakravarthula, Sarada Nallani |
| description | The aim of this study was to investigate in vitro antioxidant, anti-inflammatory and cytotoxic activities of the petroleum ether, ethyl acetate, methanol and aqueous extracts obtained from leaves of Drypetes sepiaria (Euphorbiaceae). Total phenolic and flavonoid contents of these crude extracts were determined as gallic acid and quercetin equivalents, respectively. In in vitro antioxidant method, methanol extract exhibited higher free radical scavenging activity compared to standard compound, ascorbic acid with IC₅₀ of 95.43 µg/ml (DPPH) and 67.05 µg/ml (ABTS). Methanol extract was able to inhibit inflammation by in vitro about 85-90% (HRBC stabilization method) and in vivo about 40-45% (Paw oedema method) anti-inflammatory assays compared to standard produced 50.04% at 6 h period. In cytotoxicity assay (MTT assay) methanolic extract exhibited IC₅₀ of 10 µg/ml. In apoptosis (flow cytometric assay), the control group showed normal caspase 3 activity in the SiHa cells which was 0.24%, and increased up to 40% after treatment. |
| doi_str_mv | 10.4314/ajtcam.v10i5.9 |
| format | article |
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Total phenolic and flavonoid contents of these crude extracts were determined as gallic acid and quercetin equivalents, respectively. In in vitro antioxidant method, methanol extract exhibited higher free radical scavenging activity compared to standard compound, ascorbic acid with IC₅₀ of 95.43 µg/ml (DPPH) and 67.05 µg/ml (ABTS). Methanol extract was able to inhibit inflammation by in vitro about 85-90% (HRBC stabilization method) and in vivo about 40-45% (Paw oedema method) anti-inflammatory assays compared to standard produced 50.04% at 6 h period. In cytotoxicity assay (MTT assay) methanolic extract exhibited IC₅₀ of 10 µg/ml. In apoptosis (flow cytometric assay), the control group showed normal caspase 3 activity in the SiHa cells which was 0.24%, and increased up to 40% after treatment.</description><identifier>ISSN: 0189-6016</identifier><identifier>EISSN: 2505-0044</identifier><identifier>EISSN: 0189-6016</identifier><identifier>DOI: 10.4314/ajtcam.v10i5.9</identifier><identifier>PMID: 24311836</identifier><language>eng</language><publisher>Nigeria: African Networks on Ethnomedicines</publisher><subject>Animals ; Anti-Inflammatory Agents - analysis ; Anti-Inflammatory Agents - pharmacology ; Anti-Inflammatory Agents - therapeutic use ; Antineoplastic Agents, Phytogenic - analysis ; Antineoplastic Agents, Phytogenic - pharmacology ; Antineoplastic Agents, Phytogenic - therapeutic use ; Antioxidants - analysis ; Antioxidants - pharmacology ; Antioxidants - therapeutic use ; Apoptosis ; Caspase 3 - metabolism ; Cell Line, Tumor ; Edema - chemically induced ; Edema - drug therapy ; Euphorbiaceae - chemistry ; Female ; Flavonoids - analysis ; Flavonoids - pharmacology ; Flavonoids - therapeutic use ; Humans ; Inflammation - chemically induced ; Inflammation - drug therapy ; Inhibitory Concentration 50 ; Male ; Phenols - analysis ; Phenols - pharmacology ; Phenols - therapeutic use ; Phytotherapy ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Extracts - therapeutic use ; Rats ; Rats, Wistar ; Research Papers ; Uterine Cervical Neoplasms - drug therapy</subject><ispartof>African journal of traditional, complementary, and alternative medicines, 2013-01, Vol.10 (5), p.274-282</ispartof><rights>Copyright © Afr. 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Traditional Complementary and Alternative Medicines 2013 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-7c739404886ea80de9e3ea793cf74875f14241e8be16fab2bba918c2804d15f53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847416/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847416/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24311836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gadamsetty, Ganesh</creatorcontrib><creatorcontrib>Maru, Saurabh</creatorcontrib><creatorcontrib>Tyagi, Abhishek</creatorcontrib><creatorcontrib>Chakravarthula, Sarada Nallani</creatorcontrib><title>Anti-inflammatory, cytotoxic and antioxidant effects of methanolic extracts of Drypetes sepiaria (Euphorbiaceae)</title><title>African journal of traditional, complementary, and alternative medicines</title><addtitle>Afr J Tradit Complement Altern Med</addtitle><description>The aim of this study was to investigate in vitro antioxidant, anti-inflammatory and cytotoxic activities of the petroleum ether, ethyl acetate, methanol and aqueous extracts obtained from leaves of Drypetes sepiaria (Euphorbiaceae). Total phenolic and flavonoid contents of these crude extracts were determined as gallic acid and quercetin equivalents, respectively. In in vitro antioxidant method, methanol extract exhibited higher free radical scavenging activity compared to standard compound, ascorbic acid with IC₅₀ of 95.43 µg/ml (DPPH) and 67.05 µg/ml (ABTS). Methanol extract was able to inhibit inflammation by in vitro about 85-90% (HRBC stabilization method) and in vivo about 40-45% (Paw oedema method) anti-inflammatory assays compared to standard produced 50.04% at 6 h period. In cytotoxicity assay (MTT assay) methanolic extract exhibited IC₅₀ of 10 µg/ml. In apoptosis (flow cytometric assay), the control group showed normal caspase 3 activity in the SiHa cells which was 0.24%, and increased up to 40% after treatment.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents - analysis</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Anti-Inflammatory Agents - therapeutic use</subject><subject>Antineoplastic Agents, Phytogenic - analysis</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Antineoplastic Agents, Phytogenic - therapeutic use</subject><subject>Antioxidants - analysis</subject><subject>Antioxidants - pharmacology</subject><subject>Antioxidants - therapeutic use</subject><subject>Apoptosis</subject><subject>Caspase 3 - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Edema - chemically induced</subject><subject>Edema - drug therapy</subject><subject>Euphorbiaceae - chemistry</subject><subject>Female</subject><subject>Flavonoids - analysis</subject><subject>Flavonoids - pharmacology</subject><subject>Flavonoids - therapeutic use</subject><subject>Humans</subject><subject>Inflammation - chemically induced</subject><subject>Inflammation - drug therapy</subject><subject>Inhibitory Concentration 50</subject><subject>Male</subject><subject>Phenols - analysis</subject><subject>Phenols - pharmacology</subject><subject>Phenols - therapeutic use</subject><subject>Phytotherapy</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - therapeutic use</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Research Papers</subject><subject>Uterine Cervical Neoplasms - drug therapy</subject><issn>0189-6016</issn><issn>2505-0044</issn><issn>0189-6016</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNpVUF1LwzAUDaLonL76KH1UsDNp0jZ9EYbODxB80edwm964yNqUNBvbvze6KfpwOdyPc-7hEHLG6ERwJq7hI2hoJytGbT6p9sgoy2meUirEPhlRJqu0oKw4IsfD8EEplywrD8lRFrlM8mJE-mkXbGo7s4C2heD85irRm-CCW1udQNfECjY2TcQEjUEdhsSZpMUwh84t4hWug4fd-M5vegw4JAP2FryF5GK27OfO1xY0Al6ekAMDiwFPdzgmb_ez19vH9Pnl4el2-pxqwYqQlrrklaBCygJB0gYr5AhlxbUphSxzw0QmGMoaWWGgzuoaKiZ1JqloWG5yPiY3W91-WbfYaOyiyYXqvW3Bb5QDq_5vOjtX726luBRltBAFJlsB7d0weDS_XEbVV_Zqm736zl5VkXD-9-Pv-U_Y_BMQX4Wd</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Gadamsetty, Ganesh</creator><creator>Maru, Saurabh</creator><creator>Tyagi, Abhishek</creator><creator>Chakravarthula, Sarada Nallani</creator><general>African Networks on Ethnomedicines</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20130101</creationdate><title>Anti-inflammatory, cytotoxic and antioxidant effects of methanolic extracts of Drypetes sepiaria (Euphorbiaceae)</title><author>Gadamsetty, Ganesh ; Maru, Saurabh ; Tyagi, Abhishek ; Chakravarthula, Sarada Nallani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-7c739404886ea80de9e3ea793cf74875f14241e8be16fab2bba918c2804d15f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents - analysis</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Anti-Inflammatory Agents - therapeutic use</topic><topic>Antineoplastic Agents, Phytogenic - analysis</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>Antineoplastic Agents, Phytogenic - therapeutic use</topic><topic>Antioxidants - analysis</topic><topic>Antioxidants - pharmacology</topic><topic>Antioxidants - therapeutic use</topic><topic>Apoptosis</topic><topic>Caspase 3 - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Edema - chemically induced</topic><topic>Edema - drug therapy</topic><topic>Euphorbiaceae - chemistry</topic><topic>Female</topic><topic>Flavonoids - analysis</topic><topic>Flavonoids - pharmacology</topic><topic>Flavonoids - therapeutic use</topic><topic>Humans</topic><topic>Inflammation - chemically induced</topic><topic>Inflammation - drug therapy</topic><topic>Inhibitory Concentration 50</topic><topic>Male</topic><topic>Phenols - analysis</topic><topic>Phenols - pharmacology</topic><topic>Phenols - therapeutic use</topic><topic>Phytotherapy</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - therapeutic use</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Research Papers</topic><topic>Uterine Cervical Neoplasms - drug therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gadamsetty, Ganesh</creatorcontrib><creatorcontrib>Maru, Saurabh</creatorcontrib><creatorcontrib>Tyagi, Abhishek</creatorcontrib><creatorcontrib>Chakravarthula, Sarada Nallani</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>African journal of traditional, complementary, and alternative medicines</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gadamsetty, Ganesh</au><au>Maru, Saurabh</au><au>Tyagi, Abhishek</au><au>Chakravarthula, Sarada Nallani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-inflammatory, cytotoxic and antioxidant effects of methanolic extracts of Drypetes sepiaria (Euphorbiaceae)</atitle><jtitle>African journal of traditional, complementary, and alternative medicines</jtitle><addtitle>Afr J Tradit Complement Altern Med</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>10</volume><issue>5</issue><spage>274</spage><epage>282</epage><pages>274-282</pages><issn>0189-6016</issn><eissn>2505-0044</eissn><eissn>0189-6016</eissn><abstract>The aim of this study was to investigate in vitro antioxidant, anti-inflammatory and cytotoxic activities of the petroleum ether, ethyl acetate, methanol and aqueous extracts obtained from leaves of Drypetes sepiaria (Euphorbiaceae). Total phenolic and flavonoid contents of these crude extracts were determined as gallic acid and quercetin equivalents, respectively. In in vitro antioxidant method, methanol extract exhibited higher free radical scavenging activity compared to standard compound, ascorbic acid with IC₅₀ of 95.43 µg/ml (DPPH) and 67.05 µg/ml (ABTS). Methanol extract was able to inhibit inflammation by in vitro about 85-90% (HRBC stabilization method) and in vivo about 40-45% (Paw oedema method) anti-inflammatory assays compared to standard produced 50.04% at 6 h period. In cytotoxicity assay (MTT assay) methanolic extract exhibited IC₅₀ of 10 µg/ml. In apoptosis (flow cytometric assay), the control group showed normal caspase 3 activity in the SiHa cells which was 0.24%, and increased up to 40% after treatment.</abstract><cop>Nigeria</cop><pub>African Networks on Ethnomedicines</pub><pmid>24311836</pmid><doi>10.4314/ajtcam.v10i5.9</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | African journal of traditional, complementary, and alternative medicines, 2013-01, Vol.10 (5), p.274-282 |
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| subjects | Animals Anti-Inflammatory Agents - analysis Anti-Inflammatory Agents - pharmacology Anti-Inflammatory Agents - therapeutic use Antineoplastic Agents, Phytogenic - analysis Antineoplastic Agents, Phytogenic - pharmacology Antineoplastic Agents, Phytogenic - therapeutic use Antioxidants - analysis Antioxidants - pharmacology Antioxidants - therapeutic use Apoptosis Caspase 3 - metabolism Cell Line, Tumor Edema - chemically induced Edema - drug therapy Euphorbiaceae - chemistry Female Flavonoids - analysis Flavonoids - pharmacology Flavonoids - therapeutic use Humans Inflammation - chemically induced Inflammation - drug therapy Inhibitory Concentration 50 Male Phenols - analysis Phenols - pharmacology Phenols - therapeutic use Phytotherapy Plant Extracts - chemistry Plant Extracts - pharmacology Plant Extracts - therapeutic use Rats Rats, Wistar Research Papers Uterine Cervical Neoplasms - drug therapy |
| title | Anti-inflammatory, cytotoxic and antioxidant effects of methanolic extracts of Drypetes sepiaria (Euphorbiaceae) |
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