Is the Oxidative DNA Damage Level of Human Lymphocyte Correlated with the Antioxidant Capacity of Serum or the Base Excision Repair Activity of Lymphocyte?

A random screening of human blood samples from 24 individuals of nonsmoker was conducted to examine the correlation between the oxidative DNA damage level of lymphocytes and the antioxidant capacity of serum or the base excision repair (BER) activity of lymphocytes. The oxidative DNA damage level wa...

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Bibliographic Details
Published in:Oxidative medicine and cellular longevity 2013-01, Vol.2013 (2013), p.1-8
Main Authors: Liu, Yin-Chang, Chen, Chung-Chu, Li, Pei-Yi, Tsai, Yi-Chih
Format: Article
Language:English
Subjects:
Adult
Antioxidants - metabolism
Cell Extracts
Cell Line, Tumor
Cell Nucleus - metabolism
DNA Damage
DNA Repair
Female
Helicobacter Infections - pathology
Hepatitis B - pathology
Humans
Lymphocytes - metabolism
Male
Oxidation-Reduction
Serum - metabolism
Young Adult
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Summary:A random screening of human blood samples from 24 individuals of nonsmoker was conducted to examine the correlation between the oxidative DNA damage level of lymphocytes and the antioxidant capacity of serum or the base excision repair (BER) activity of lymphocytes. The oxidative DNA damage level was measured with comet assay containing Fpg/Endo III cleavage, and the BER activity was estimated with a modified comet assay including nuclear extract of lymphocytes for enzymatic cleavage. Antioxidant capacity was determined with trolox equivalent antioxidant capacity assay. We found that though the endogenous DNA oxidation levels varied among the individuals, each individual level appeared to be steady for at least 1 month. Our results indicate that the oxidative DNA damage level is insignificantly or weakly correlated with antioxidant capacity or BER activity, respectively. However, lymphocytes from carriers of Helicobacter pylori (HP) or Hepatitis B virus (HBV) tend to give higher levels of oxidative DNA damage (P
ISSN:1942-0900
1942-0994
DOI:10.1155/2013/237583