Dual mechanisms prevent premature chromosome segregation during meiosis

In meiosis I, homologous chromosomes pair and then attach to the spindle so that the homologs can be pulled apart at anaphase I. The segregation of homologs before pairing would be catastrophic. We describe two mechanisms that prevent this. First, in early meiosis, Ipl1, the budding yeast homolog of...

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Bibliographic Details
Published in:Genes & development 2013-10, Vol.27 (19), p.2139-2146
Main Authors: Kim, Seoyoung, Meyer, Régis, Chuong, Hoa, Dawson, Dean S
Format: Article
Language:English
Subjects:
Aurora Kinases - genetics
Aurora Kinases - metabolism
Chromosome Segregation - genetics
Chromosome Segregation - physiology
Cyclin-Dependent Kinases - metabolism
DNA-Binding Proteins - metabolism
Gene Expression Regulation, Fungal
Kinetochores - metabolism
Meiosis - genetics
Meiosis - physiology
Microtubules - metabolism
Phosphorylation
Protein Transport
Research Paper
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Spindle Apparatus - metabolism
Transcription Factors - metabolism
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Summary:In meiosis I, homologous chromosomes pair and then attach to the spindle so that the homologs can be pulled apart at anaphase I. The segregation of homologs before pairing would be catastrophic. We describe two mechanisms that prevent this. First, in early meiosis, Ipl1, the budding yeast homolog of the mammalian Aurora B kinase, triggers shedding of a kinetochore protein, preventing microtubule attachment. Second, Ipl1 localizes to the spindle pole bodies (SPBs), where it blocks spindle assembly. These processes are reversed upon expression of Ndt80. Previous studies have shown that Ndt80 is expressed when homologs have successfully partnered, and this triggers a rise in the levels of cyclin-dependent kinase (CDK). We found that CDK phosphorylates Ipl1, delocalizing it from SPBs, triggering spindle assembly. At the same time, kinetochores reassemble. Thus, dual mechanisms controlled by Ipl1 and Ntd80 coordinate chromosome and spindle behaviors to prevent the attachment of unpartnered chromosomes to the meiotic spindle.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.227454.113