Monoclonal antibody recognizing a core epitope on mucin

Monoclonal antibody TH1 (IgM) was prepared by immunizing mice with deglycosylated (TFMSA-treated) cystic fibrosis mucin. TH1 reacted strongly with TFMSA treated cystic fibrosis mucin but not with the fully glycosylated mucin, indicating reactivity with a core mucin epitope. TH1 showed no reactivity...

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Bibliographic Details
Published in:Disease markers 1998-10, Vol.14 (2), p.99-112
Main Authors: DEVINE, P. L, QUIN, R. J, SHIELD, P. W, YEW WAH LIEW, SHEEHAN, J. K, THORNTON, D. J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - immunology
Antibody Specificity
Biological and medical sciences
Breast Neoplasms - metabolism
Colonic Neoplasms - metabolism
Cystic Fibrosis - metabolism
Enzyme-Linked Immunosorbent Assay
Epitopes - immunology
Female
Fundamental and applied biological sciences. Psychology
Glycoproteins
Glycosylation
Hybridomas - immunology
Immunoenzyme Techniques
Mesylates - pharmacology
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Mucins - analysis
Mucins - chemistry
Mucins - immunology
Neoplasms - chemistry
Other
Ovarian Neoplasms - metabolism
Proteins
Tumor Cells, Cultured
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Summary:Monoclonal antibody TH1 (IgM) was prepared by immunizing mice with deglycosylated (TFMSA-treated) cystic fibrosis mucin. TH1 reacted strongly with TFMSA treated cystic fibrosis mucin but not with the fully glycosylated mucin, indicating reactivity with a core mucin epitope. TH1 showed no reactivity with ovine mucin (98% of glycans as sialyl-Tn) but reacted strongly with desialylated ovine mucin, indicating the epitope for this mab was the Tn-antigen (O-linked GalNAc). However, TH1 showed no reactivity with Tn-positive red blood cells, and the binding of TH1 was not inhibited by GalNAc at 2.5 mg/ml, illustrating the importance of the peptide sequence to which the GalNAc is attached. TH1 stained the majority of cancers of the colon, lung, stomach, ovary, breast, and cervix, and the cellular distribution of this antigen in normal tissue suggested reactivity with immature mucin. This antibody appears to be a useful reagent for the detection of immature mucin.
ISSN:0278-0240
1875-8630
DOI:10.1155/1998/678434