Gene expression profile in circulating mononuclear cells after exposure to ultrafine carbon particles

• Published in: Inhalation toxicology 2010-08, Vol.22 (10), p.835-846
• Main Authors: Huang, Yuh-Chin T., Schmitt, Michael, Yang, Zhonghui, Que, Loretta G., Stewart, Judith C., Frampton, Mark W., Devlin, Robert B.
• Format: Article
• Language: English
• Subjects: Adult; Air Pollutants - toxicity; Carbon - toxicity; Female; Gene expression; Gene Expression - drug effects; Gene Expression Profiling; glucose metabolism; Humans; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - metabolism; Male; microarray; Oligonucleotide Array Sequence Analysis; oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - genetics; Particle Size; particulate matter; ultrafine particles; Up-Regulation - drug effects; Young Adult
• ISSN: 0895-8378; 1091-7691
• DOI: 10.3109/08958378.2010.486419

Context: Exposure to particulate matter (PM) is associated with systemic health effects, but the cellular and molecular mechanisms are unclear. Objective: We hypothesized that, if circulating mononuclear cells play an important role in mediating systemic effects of PM, they would show gene expression changes following exposure. Materials and methods: Peripheral blood samples were collected before (0 h) and at 24 h from healthy subjects exposed to filtered air (FA) and ultrafine carbon particles (UFPs, 50 μg/m3) for 2 h in a previous study (n = 3 each). RNA from mononuclear cell fraction (>85% lymphocytes) was extracted, amplified and hybridized to Affymetrix HU133 plus 2 microarrays. Selected genes were confirmed in five additional subjects from the same study. Results: We identified 1713 genes (UFP 24 h vs. FA 0 and 24 h, P 1.1 or