Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons

Upregulation of small heat-shock proteins (sHsps) in response to cellular stress is one mechanism to increase cell viability. We previously described that cultured rat hippocampal neurons express five of the 11 family members but only upregulate two of them (HspB1 and HspB5) at the protein level aft...

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Bibliographic Details
Published in:Cell stress & chaperones 2014, Vol.19 (1), p.145-158
Main Authors: Bartelt-Kirbach, Britta, Golenhofen, Nikola
Format: Article
Language:English
Subjects:
Animals
Antibodies
Arsenites
Arsenites - pharmacology
Biochemistry
Biomedical and Life Sciences
Biomedicine
Brain
Cancer Research
Cell Biology
Cells, Cultured
Enzyme Inhibitors - pharmacology
Heat-Shock Proteins, Small - genetics
Heat-Shock Proteins, Small - metabolism
Hippocampus - cytology
Hippocampus - metabolism
Immunology
Messenger RNA
Neurons
Neurosciences
Osmotic Pressure
Oxidative Stress
Rats
RNA, Messenger - metabolism
Shock heating
SHORT COMMUNICATION
Small heat shock proteins
Sodium
Sodium Compounds - pharmacology
Up regulation
Up-Regulation - drug effects
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Summary:Upregulation of small heat-shock proteins (sHsps) in response to cellular stress is one mechanism to increase cell viability. We previously described that cultured rat hippocampal neurons express five of the 11 family members but only upregulate two of them (HspB1 and HspB5) at the protein level after heat stress. Since neurons have to cope with many other pathological conditions, we investigated in this study the expression of all five expressed sHsps on mRNA and protein level after sublethal sodium arsenite and oxidative and hyperosmotic stress. Under all three conditions, HspB1, HspB5, HspB6, and HspB8 but not HspB11 were consistently upregulated but showed differences in the time course of upregulation. The increase of sHsps always occurred earlier on mRNA level compared with protein levels. We conclude from our data that these four upregulated sHsps (HspB1, HspB5, HspB6, HspB8) act together in different proportions in the protection of neurons from various stress conditions.
ISSN:1355-8145
1466-1268
DOI:10.1007/s12192-013-0452-9