Increased bioplastic production with an RNA polymerase sigma factor SigE during nitrogen starvation in Synechocystis sp. PCC 6803

Because cyanobacteria directly harvest CO2 and light energy, their carbon metabolism is important for both basic and applied sciences. Here, we show that overexpression of the sigma factor sigE in Synechocystis sp. PCC 6803 widely changes sugar catabolism and increases production of the biodegradabl...

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Bibliographic Details
Published in:DNA research 2013-12, Vol.20 (6), p.525-535
Main Authors: Osanai, Takashi, Numata, Keiji, Oikawa, Akira, Kuwahara, Ayuko, Iijima, Hiroko, Doi, Yoshiharu, Tanaka, Kan, Saito, Kazuki, Hirai, Masami Yokota
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Cyanobacteria
Glycogen - genetics
Glycogen - metabolism
Nitrogen - metabolism
Polyhydroxyalkanoates - biosynthesis
Sigma Factor - genetics
Sigma Factor - metabolism
Synechocystis
Synechocystis - genetics
Synechocystis - metabolism
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Summary:Because cyanobacteria directly harvest CO2 and light energy, their carbon metabolism is important for both basic and applied sciences. Here, we show that overexpression of the sigma factor sigE in Synechocystis sp. PCC 6803 widely changes sugar catabolism and increases production of the biodegradable polyester polyhydroxybutyrate (PHB) during nitrogen starvation. sigE overexpression elevates the levels of proteins implicated in glycogen catabolism, the oxidative pentose phosphate pathway, and polyhydroxyalkanoate biosynthesis. PHB accumulation is enhanced by sigE overexpression under nitrogen-limited conditions, yet the molecular weights of PHBs synthesized by the parental glucose-tolerant and sigE overexpression strain are similar. Although gene expression induced by nitrogen starvation is changed and other metabolites (such as GDP-mannose and citrate) accumulate under sigE overexpression, genetic engineering of this sigma factor altered the metabolic pathway from glycogen to PHB during nitrogen starvation.
ISSN:1340-2838
1756-1663
DOI:10.1093/dnares/dst028