Identification of a Paternal Developmental Effect on the Cytoplasm of One-Cell-Stage Mouse Embryos

Matings of female DDK mice with males of the BALB/c strain are sterile, whereas reciprocal crosses are normally fertile. We used nuclear transplantation between the hybrid eggs of these two strains to investigate the basis of this effect. We demonstrate that the observed sterility results from early...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-09, Vol.83 (18), p.6883-6886
Main Authors: Renard, J. P., Ch. Babinet
Format: Article
Language:English
Subjects:
Animals
Blastocyst
Cell Nucleus - physiology
Crosses, Genetic
Cytoplasm
Cytoplasm - physiology
Embryos
Female
Female animals
Fertilization
Genotypes
Infertility - etiology
Male
Male animals
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Ova
Pronucleus
Spermatozoa
Zygote - cytology
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Summary:Matings of female DDK mice with males of the BALB/c strain are sterile, whereas reciprocal crosses are normally fertile. We used nuclear transplantation between the hybrid eggs of these two strains to investigate the basis of this effect. We demonstrate that the observed sterility results from early embryonic mortality, that the mortality is due to a modification of the egg cytoplasm, and that the modification is mediated by the male pronucleus. Once established, this modification may affect female pronuclei of unrelated genotype such as C57BL/6. These results support the notion that a product derived from the male genome acts at the pronuclear stage and can affect later stages of embryonic development.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.18.6883