Proteomics profiling reveals novel proteins and functions of the plant stigma exudate

• Published in: Journal of experimental botany 2013-12, Vol.64 (18), p.5695-5705
• Main Authors: Rejón, Juan David, Delalande, François, Schaeffer-Reiss, Christine, Carapito, Christine, Zienkiewicz, Krzysztof, de Dios Alché, Juan, Rodríguez-García, María Isabel, Van Dorsselaer, Alain, Castro, Antonio Jesús
• Format: Article
• Language: English
• Subjects: adhesion; apoptosis; Biological and medical sciences; Catabolism; Cell Wall - metabolism; Enzymes; Flowers - chemistry; Flowers - metabolism; Fundamental and applied biological sciences. Psychology; Gels; Lilium - chemistry; Lilium - metabolism; Lilium longiflorum; lipids; Olea - chemistry; Olea - metabolism; Olea europaea; Olives; pistil; Plant cells; Plant Exudates - chemistry; Plant Exudates - metabolism; Plant physiology and development; Plant Proteins - analysis; Plant Proteins - metabolism; Plants; Pollen; Pollen Tube - growth & development; Pollen tubes; Pollination; polysaccharides; Polysaccharides - metabolism; proteins; proteomics; Proteomics - methods; RESEARCH PAPER; stigma; Trucks
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/ert345

Proteomic analysis of the stigmatic exudate of Lilium longiflorum and Olea europaea led to the identification of 51 and 57 proteins, respectively, most of which are described for the first time in this secreted fluid. These results indicate that the stigmatic exudate is an extracellular environment metabolically active, participating in at least 80 different biological processes and 97 molecular functions. The stigma exudate showed a markedly catabolic profile and appeared to possess the enzyme machinery necessary to degrade large polysaccharides and lipids secreted by papillae to smaller units, allowing their incorporation into the pollen tube during pollination. It may also regulate pollen-tube growth in the pistil through the selective degradation of tube-wall components. Furthermore, some secreted proteins were involved in pollen-tube adhesion and orientation, as well as in programmed cell death of the papillae cells in response to either compatible pollination or incompatible pollen rejection. Finally, the results also revealed a putative cross-talk between genetic programmes regulating stress/defence and pollination responses in the stigma.