The effect of beta-elemene on alpha-tubulin polymerization in human hepatoma HepG2 cells

Objective： To investigate the impact of beta-elemene injection on the growth and alpha-tubule of human hepatocarcinoma HepG2 cells. Methods： Cell proliferation was assessed by MTT assay. Cell cycle distribution was detected by flow cytometry （FCM）. The mRNA expression of alpha-tubulin was measured b...

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Published in:Chinese journal of cancer research 2013-12, Vol.25 (6), p.770-776
Main Authors: Mao, Yuqiu, Zhang, Jielin, Hou, Li, Cui, Xiaonan
Format: Article
Language:English
Subjects:
HepG2细胞
Original
RT-PCR检测
Western印迹
α-微管蛋白
β-榄香烯
时间依赖性
聚合
肝癌
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creator Mao, Yuqiu
Zhang, Jielin
Hou, Li
Cui, Xiaonan
description Objective： To investigate the impact of beta-elemene injection on the growth and alpha-tubule of human hepatocarcinoma HepG2 cells. Methods： Cell proliferation was assessed by MTT assay. Cell cycle distribution was detected by flow cytometry （FCM）. The mRNA expression of alpha-tubulin was measured by RT-PCR. Western blot analysis was used to determine protein expression of alpha-tubulin and the polymerization of tubulin. Results： Beta-elemene injection inhibited HepG2 cells proliferation in a dose- and time-dependent manner; FCM analysis indicated beta-elemene injection induced cell cycle arrested at S phase. RT-PCR and western- blot analysis showed that beta-elemene injection down-regulated alpha-tublin at both mRNA and protein levels, presenting a dose-dependent manner. Moreover, beta-elemene injection reduced the polymerization of microtubules in a dose-dependent manner. Conclusions： Beta-elemene injection can inhibit the proliferation of hepatoma HepG2 cells and induce cell apoptosis, the mechanism might be partly related to the down-regulation of alpha-tubulin and inhibition of microtubular polymerization.
doi_str_mv 10.3978/j.issn.1000-9604.2013.12.12
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Methods： Cell proliferation was assessed by MTT assay. Cell cycle distribution was detected by flow cytometry （FCM）. The mRNA expression of alpha-tubulin was measured by RT-PCR. Western blot analysis was used to determine protein expression of alpha-tubulin and the polymerization of tubulin. Results： Beta-elemene injection inhibited HepG2 cells proliferation in a dose- and time-dependent manner; FCM analysis indicated beta-elemene injection induced cell cycle arrested at S phase. RT-PCR and western- blot analysis showed that beta-elemene injection down-regulated alpha-tublin at both mRNA and protein levels, presenting a dose-dependent manner. Moreover, beta-elemene injection reduced the polymerization of microtubules in a dose-dependent manner. Conclusions： Beta-elemene injection can inhibit the proliferation of hepatoma HepG2 cells and induce cell apoptosis, the mechanism might be partly related to the down-regulation of alpha-tubulin and inhibition of microtubular polymerization.</description><identifier>ISSN: 1000-9604</identifier><identifier>EISSN: 1993-0631</identifier><identifier>DOI: 10.3978/j.issn.1000-9604.2013.12.12</identifier><identifier>PMID: 24385707</identifier><language>eng</language><publisher>China: Department of Oncology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China%Department of Pathology, Dalian Medical University, Dalian 116027, China</publisher><subject>HepG2细胞 ; Original ; RT-PCR检测 ; Western印迹 ; α-微管蛋白 ; β-榄香烯 ; 时间依赖性 ; 聚合 ; 肝癌</subject><ispartof>Chinese journal of cancer research, 2013-12, Vol.25 (6), p.770-776</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2013 Chinese Journal of Cancer Research. 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Methods： Cell proliferation was assessed by MTT assay. Cell cycle distribution was detected by flow cytometry （FCM）. The mRNA expression of alpha-tubulin was measured by RT-PCR. Western blot analysis was used to determine protein expression of alpha-tubulin and the polymerization of tubulin. Results： Beta-elemene injection inhibited HepG2 cells proliferation in a dose- and time-dependent manner; FCM analysis indicated beta-elemene injection induced cell cycle arrested at S phase. RT-PCR and western- blot analysis showed that beta-elemene injection down-regulated alpha-tublin at both mRNA and protein levels, presenting a dose-dependent manner. Moreover, beta-elemene injection reduced the polymerization of microtubules in a dose-dependent manner. 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subjects HepG2细胞
Original
RT-PCR检测
Western印迹
α-微管蛋白
β-榄香烯
时间依赖性
聚合
肝癌
title The effect of beta-elemene on alpha-tubulin polymerization in human hepatoma HepG2 cells
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