The immediate upstream region of the 5'-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana

The nucleotide sequence around the translational initiation site is an important cis-acting element for post-transcriptional regulation. However, it has not been fully understood how the sequence context at the 5'-untranslated region (5'-UTR) affects the translational efficiency of individ...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 2014-01, Vol.42 (1), p.485-498
Main Authors: Kim, Younghyun, Lee, Goeun, Jeon, Eunhyun, Sohn, Eun Ju, Lee, Yongjik, Kang, Hyangju, Lee, Dong Wook, Kim, Dae Heon, Hwang, Inhwan
Format: Article
Language:English
Subjects:
5' Untranslated Regions
Adenine - chemistry
Arabidopsis - genetics
Base Sequence
Codon, Initiator
Genes, Plant
Molecular Biology
Protein Biosynthesis
RNA, Messenger - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The nucleotide sequence around the translational initiation site is an important cis-acting element for post-transcriptional regulation. However, it has not been fully understood how the sequence context at the 5'-untranslated region (5'-UTR) affects the translational efficiency of individual mRNAs. In this study, we provide evidence that the 5'-UTRs of Arabidopsis genes showing a great difference in the nucleotide sequence vary greatly in translational efficiency with more than a 200-fold difference. Of the four types of nucleotides, the A residue was the most favourable nucleotide from positions -1 to -21 of the 5'-UTRs in Arabidopsis genes. In particular, the A residue in the 5'-UTR from positions -1 to -5 was required for a high-level translational efficiency. In contrast, the T residue in the 5'-UTR from positions -1 to -5 was the least favourable nucleotide in translational efficiency. Furthermore, the effect of the sequence context in the -1 to -21 region of the 5'-UTR was conserved in different plant species. Based on these observations, we propose that the sequence context immediately upstream of the AUG initiation codon plays a crucial role in determining the translational efficiency of plant genes.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkt864