Protective response to subunit vaccination against intranasal Burkholderia mallei and B. pseudomallei challenge

Burkholderia mallei and B. pseudomallei are Gram-negative pathogenic bacteria, responsible for the diseases glanders and melioidosis, respectively. Furthermore, there is currently no vaccine available against these Burkholderia species. In this study, we aimed to identify protective proteins against...

Full description

Saved in:
Bibliographic Details
Published in:Procedia in vaccinology 2010, Vol.2 (1), p.73-77
Main Authors: Whitlock, Gregory C., Deeraksa, Arpaporn, Qazi, Omar, Judy, Barbara M., Taylor, Katherine, Propst, Katie L., Duffy, Angie J., Johnson, Kate, Kitto, G. Barrie, Brown, Katherine A., Dow, Steven W., Torres, Alfredo G., Estes, D. Mark
Format: Article
Language:English
Subjects:
Bordetella pertussis
BPZE1
BrkA autotransporter
Enterovirus 71
Live mucosal vaccine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Burkholderia mallei and B. pseudomallei are Gram-negative pathogenic bacteria, responsible for the diseases glanders and melioidosis, respectively. Furthermore, there is currently no vaccine available against these Burkholderia species. In this study, we aimed to identify protective proteins against these pathogens. Immunization with recombinant B. mallei Hcp1 (type VI secreted/structural protein), BimA (autotransporter protein), BopA (type III secreted protein), and B. pseudomallei LolC (ABC transporter protein) generated significant protection against lethal inhaled B. mallei ATCC23344 and B. pseudomallei 1026b challenge. Immunization with BopA elicited the greatest protective activity, resulting in 100% and 60% survival against B. mallei and B. pseudomallei challenge, respectively. Moreover, sera from recovered mice demonstrated reactivity with the recombinant proteins. Dendritic cells stimulated with each of the different recombinant proteins showed distinct cytokine patterns. In addition, T cells from immunized mice produced IFN-γ following in vitro re-stimulation. These results indicated therefore that it was possible to elicit cross-protective immunity against both B. mallei and B. pseudomallei by vaccinating animals with one or more novel recombinant proteins identified in B. mallei.
ISSN:1877-282X
1877-282X
DOI:10.1016/j.provac.2010.03.013