Preparative Purification of Recombinant Proteins: Current Status and Future Trends

Advances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification...

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Bibliographic Details
Published in:BioMed research international 2013-01, Vol.2013 (2013), p.1-18
Main Authors: Byrne, Barry, Shortt, Brian, Ravidá, Alessandra, Musante, Luca, Saraswat, Mayank, Holthofer, Harry
Format: Article
Language:English
Subjects:
Animals
Chromatography
Chromatography - classification
Chromatography - methods
Chromatography - trends
Costs
Humans
Medical research
Pharmaceutical industry
Production processes
Proteins
Recombinant proteins
Recombinant Proteins - chemistry
Recombinant Proteins - isolation & purification
Review
Studies
Trends
Water - chemistry
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Summary:Advances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products in the absence of contaminants, such as superfluous proteins and endotoxins. Here, we provide a comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings and discuss how innovative and effective protocols such as aqueous two-phase partitioning, membrane chromatography, and high-performance tangential flow filtration may be applied independently of or in conjunction with more traditional protocols for downstream processing applications.
ISSN:2314-6133
2314-6141
DOI:10.1155/2013/312709