Overexpression of kallikrein gene 10 is a biomarker for predicting poor prognosis in gastric cancer

• Published in: World journal of gastroenterology : WJG 2013-12, Vol.19 (48), p.9425-9431
• Main Authors: Jiao, Xin, Lu, Hong-Jun, Zhai, Mi-Mi, Tan, Zhi-Jun, Zhi, Hai-Ning, Liu, Xiao-Man, Liu, Chen-Hao, Zhang, Da-Peng
• Format: Article
• Language: English
• Subjects: Adult; Aged; Biomarkers, Tumor - analysis; Biomarkers, Tumor - genetics; Brief; Chi-Square Distribution; Disease-Free Survival; Female; Humans; Immunohistochemistry; Kallikreins - analysis; Kallikreins - genetics; Kaplan-Meier Estimate; Lymphatic Metastasis; Male; Middle Aged; Multivariate Analysis; Neoplasm Invasiveness; Predictive Value of Tests; Proportional Hazards Models; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Risk Factors; RNA, Messenger - analysis; Stomach Neoplasms - enzymology; Stomach Neoplasms - genetics; Stomach Neoplasms - mortality; Stomach Neoplasms - pathology; Stomach Neoplasms - therapy; Time Factors; Treatment Outcome; Up-Regulation
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v19.i48.9425

To analyze the expression of kallikrein gene 10 (KLK10) in gastric cancer and to determine whether KLK10 has independent prognostic value in gastric cancer. We studied KLK10 expression in 80 histologically confirmed gastric cancer samples using real-time quantitative reverse transcription-PCR and hK10 expression using immunohistochemistry. Correlations with clinicopathological variables (lymph node metastasis, depth of invasion and histology) and with outcomes (disease-free survival and overall survival) during a median follow-up period of 31 mo were assessed. Gastric cancer tissues were then classified as KLK10 positive or negative. KLK10 was found to be highly expressed in 57/80 (70%) of gastric cancer samples, while its expression was very low in normal gastric tissues. Positive relationships between KLK10 expression and lymph node metastasis (P = 0.048), depth of invasion (P = 0.034) and histology (P = 0.015) were observed. Univariate survival analysis revealed that gastric cancer patients with positive KLK10 expression had an increased risk for relapse/metastasis and death (P = 0.005 and 0.002, respectively). Cox multivariate analysis indicated that KLK10 was an independent prognostic indicator of disease-free survival and overall survival in patients with gastric cancer. KLK10 expression is an independent biomarker of unfavorable prognosis in patients with gastric cancer.