Genotypically Different Clones of Staphylococcus aureus Are Diverse in the Antimicrobial Susceptibility Patterns and Biofilm Formations

This study evaluated whether genotypically different clinical isolates of S. aureus have similar susceptibilities to individual antibiotics. It further aims to check the impact of biofilm on the in vitro activity of vancomycin, daptomycin, linezolid, and tigecycline against S. aureus clones. The stu...

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Bibliographic Details
Published in:BioMed research international 2013-01, Vol.2013 (2013), p.1-10
Main Authors: Nor Shamsudin, Mariana, Hamat, Rukman Awang, Ghaznavi-Rad, Ehsanollah, Nathan, Jayakayatri Jeevajothi, Karunanidhi, Arunkumar, Pei Pei, Chong, Sekawi, Zamberi, Thian Lung, Leslie Than, Shamsudin, Mariana Nor, Atshan, Salman Sahab, Mateg Ali, Alreshidi
Format: Article
Language:English
Subjects:
Acetamides - pharmacology
Anti-Infective Agents - pharmacology
Bacteriology
Biofilms
Biofilms - growth & development
Cloning
Daptomycin - pharmacology
Drug resistance in microorganisms
Genetic aspects
Genetic Variation
Genotype
Humans
Linezolid
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - genetics
Methicillin-Resistant Staphylococcus aureus - growth & development
Microbial Viability - drug effects
Microbiological research
Minocycline - analogs & derivatives
Minocycline - pharmacology
Oxazolidinones - pharmacology
Science
Staphylococcal Infections - drug therapy
Staphylococcal Infections - genetics
Staphylococcus aureus
Staphylococcus infections
Tigecycline
Vancomycin - pharmacology
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Summary:This study evaluated whether genotypically different clinical isolates of S. aureus have similar susceptibilities to individual antibiotics. It further aims to check the impact of biofilm on the in vitro activity of vancomycin, daptomycin, linezolid, and tigecycline against S. aureus clones. The study used a total of 60 different clinical MSSA and MRSA isolates. Susceptibilities were performed in planktonic cultures by macrobroth dilution and epsilon-test (E test) system. Biofilm production was determined using an adherent plate assay. The efficacy of antimicrobial activities against biofilms formation was checked using confocal laser scanning microscopy (CLSM). The study found that similar and different spa, MLST, and SCCmec types displayed high variation in their susceptibilities to antibiotics with tigecycline and daptomycin being the most effective. The biofilms were found resistant to high concentrations of most antibiotics tested with daptomycin being the most effective drug used in adhesive biofilms. A considerable difference exists among similar and various clone types against antibiotics tested. This variation could have contributed to the degree of virulence even within the same clonal genotype and enhanced heterogeneity in the infection potential. Thus, the development of a rapid and precise identification profile for each clone in human infections is important.
ISSN:2314-6133
2314-6141
DOI:10.1155/2013/515712