Bovine in vitro fertilization: In vitro oocyte maturation and sperm capacitation with heparin

As a result of research in the 1980s on in vitro maturation, sperm capacitation, and in vitro fertilization, the bovine is now one of the important models for development. Further, the current production of bovine embryos in vitro rivals that of in vivo embryo production for commercial applications....

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Bibliographic Details
Published in:Theriogenology 2014-01, Vol.81 (1), p.67-73
Main Author: Parrish, John J.
Format: Article
Language:English
Subjects:
Animals
Bovine
Capacitation
Cattle
Cryopreservation
Female
Fertilization
Fertilization in Vitro - methods
Fertilization in Vitro - veterinary
Fibrinolytic Agents - pharmacology
Fibrinolytic Agents - therapeutic use
Heparin
Heparin - pharmacology
Heparin - therapeutic use
In Vitro Oocyte Maturation Techniques - veterinary
IVF
Male
Oocytes - growth & development
Sperm Capacitation - drug effects
Spermatozoa - drug effects
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Summary:As a result of research in the 1980s on in vitro maturation, sperm capacitation, and in vitro fertilization, the bovine is now one of the important models for development. Further, the current production of bovine embryos in vitro rivals that of in vivo embryo production for commercial applications. Researchers of today may be unaware of why decisions were made in the procedures. This review addresses the state of the art at the time of the work by Parrish et al. (Bovine in vitro fertilization with frozen thawed semen. Theriogenology 1986;25:591–600), and how later work would explain success or failure of competing procedures. Important was the use of frozen semen and heparin capacitation, because this allowed future researchers/practitioners to change sperm numbers and capacitation conditions to adjust for variations among bulls. The large numbers of citation of the original work stand the testament of time in the repeatability and success of the procedures. The work was done within the environment of the N.L. First laboratory and the unique interactions with a large number of talented graduate students, postdoctoral researchers, and technicians.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2013.08.005