Identification of candidate circulating cisplatin-resistant biomarkers from epithelial ovarian carcinoma cell secretomes

Background: The majority of patients diagnosed with advanced epithelial ovarian carcinoma (EOC) relapse with resistant disease, and there are no biomarkers that possess clinical utility to identify or monitor these patients. This study aimed to identify secreted proteins (‘secretome’) collected from...

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Bibliographic Details
Published in:British journal of cancer 2014-01, Vol.110 (1), p.123-132
Main Authors: Teng, P-N, Wang, G, Hood, B L, Conrads, K A, Hamilton, C A, Maxwell, G L, Darcy, K M, Conrads, T P
Format: Article
Language:English
Subjects:
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692/699/67/1059/2326
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Apoptosis
Biological and medical sciences
Biomarkers
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - blood
Biomarkers, Tumor - genetics
Biomedical and Life Sciences
Biomedicine
Cancer Research
Cancer therapies
Carcinoma, Ovarian Epithelial
Cell Line, Tumor
Chemotherapy
Chromatography
Cisplatin - pharmacology
Collagen Type XI - biosynthesis
Collagen Type XI - blood
Collagen Type XI - genetics
Culture Media
Disease-Free Survival
Drug Resistance
Drug Resistance, Neoplasm
Epidemiology
Female
Female genital diseases
Gene Expression
Gynecology
Gynecology. Andrology. Obstetrics
Humans
Mass spectrometry
Medical research
Medical sciences
Molecular Diagnostics
Molecular Medicine
Multiple tumors. Solid tumors. Tumors in childhood (general aspects)
Neoplasm Proteins - biosynthesis
Neoplasm Proteins - blood
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
Neoplasms, Glandular and Epithelial - blood
Neoplasms, Glandular and Epithelial - drug therapy
Neoplasms, Glandular and Epithelial - genetics
Neoplasms, Glandular and Epithelial - metabolism
Obstetrics
Oncology
Ovarian cancer
Ovarian Neoplasms - blood
Ovarian Neoplasms - drug therapy
Ovarian Neoplasms - genetics
Ovarian Neoplasms - metabolism
Patients
Proteins
Proteomics
Scientific imaging
Survival Rate
Tumors
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Summary:Background: The majority of patients diagnosed with advanced epithelial ovarian carcinoma (EOC) relapse with resistant disease, and there are no biomarkers that possess clinical utility to identify or monitor these patients. This study aimed to identify secreted proteins (‘secretome’) collected from human EOC cell lines that differ in their inherent platinum sensitivity. Methods: Secreted proteins collected from conditioned medium from ovarian cancer cell lines that vary in their sensitivity to cisplatin were digested with trypsin and analysed by liquid chromatography-tandem mass spectrometry for peptide identification. Results: Of the 1688 proteins identified, 16 possessed significant differential abundances ( P
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.2013.687