Transcription of the Polyoma Virus Genome: Synthesis and Cleavage of Giant Late Polyoma-Specific RNA

The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized ``late'' polyoma-specific RNA appears as ``giant'' molecules of he...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1971-09, Vol.68 (9), p.2231-2235
Main Authors: Acheson, Nicholas H., Buetti, Elena, Scherrer, Klaus, Weil, Roger
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Biological Sciences: Biochemistry
Carbon Isotopes
Cells, Cultured
Centrifugation, Density Gradient
DNA
Electrophoresis
Electrophoresis, Disc
Gels
Genetic Code
Infections
Kidney
Kidney cells
Mice
Molecular Weight
Molecules
Nucleic Acid Hybridization
Polyomavirus - metabolism
Ribosomal RNA
RNA
RNA - biosynthesis
RNA, Viral - analysis
RNA, Viral - biosynthesis
Tritium
Uridine - metabolism
Viruses
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Summary:The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized ``late'' polyoma-specific RNA appears as ``giant'' molecules of heterogeneous size, up to several times larger than a strand of polyoma DNA (1.5 × 106daltons). Treatment with dimethylsulfoxide or urea showed that the large size of these molecules is not due to aggregation. Giant polyoma-specific RNA is strikingly similar in size distribution to ``nuclear messenger-like'' RNA (``heterogeneous nuclear'' RNA) of the host cell. Subsequent to its synthesis, some of the giant polyoma-specific RNA appears to be cleaved to at least three smaller species.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.68.9.2231