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Transcription of the Polyoma Virus Genome: Synthesis and Cleavage of Giant Late Polyoma-Specific RNA

The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized ``late'' polyoma-specific RNA appears as ``giant'' molecules of he...

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Published in:	Proceedings of the National Academy of Sciences - PNAS 1971-09, Vol.68 (9), p.2231-2235
Main Authors:	Acheson, Nicholas H., Buetti, Elena, Scherrer, Klaus, Weil, Roger
Format:	Article
Language:	English
Subjects:	Animals Autoradiography Biological Sciences: Biochemistry Carbon Isotopes Cells, Cultured Centrifugation, Density Gradient DNA Electrophoresis Electrophoresis, Disc Gels Genetic Code Infections Kidney Kidney cells Mice Molecular Weight Molecules Nucleic Acid Hybridization Polyomavirus - metabolism Ribosomal RNA RNA RNA - biosynthesis RNA, Viral - analysis RNA, Viral - biosynthesis Tritium Uridine - metabolism Viruses
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container_issue	9
container_start_page	2231
container_title	Proceedings of the National Academy of Sciences - PNAS
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creator	Acheson, Nicholas H. Buetti, Elena Scherrer, Klaus Weil, Roger
description	The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized ``late'' polyoma-specific RNA appears as ``giant'' molecules of heterogeneous size, up to several times larger than a strand of polyoma DNA (1.5 × 106daltons). Treatment with dimethylsulfoxide or urea showed that the large size of these molecules is not due to aggregation. Giant polyoma-specific RNA is strikingly similar in size distribution to ``nuclear messenger-like'' RNA (``heterogeneous nuclear'' RNA) of the host cell. Subsequent to its synthesis, some of the giant polyoma-specific RNA appears to be cleaved to at least three smaller species.
doi_str_mv	10.1073/pnas.68.9.2231
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source	Open Access: PubMed Central; JSTOR Archival Journals and Primary Sources Collection
subjects	Animals Autoradiography Biological Sciences: Biochemistry Carbon Isotopes Cells, Cultured Centrifugation, Density Gradient DNA Electrophoresis Electrophoresis, Disc Gels Genetic Code Infections Kidney Kidney cells Mice Molecular Weight Molecules Nucleic Acid Hybridization Polyomavirus - metabolism Ribosomal RNA RNA RNA - biosynthesis RNA, Viral - analysis RNA, Viral - biosynthesis Tritium Uridine - metabolism Viruses
title	Transcription of the Polyoma Virus Genome: Synthesis and Cleavage of Giant Late Polyoma-Specific RNA
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