Measuring single cell mass, volume, and density with dual suspended microchannel resonators

Cell size, measured as either volume or mass, is a fundamental indicator of cell state. Far more tightly regulated than size is density, the ratio between mass and volume, which can be used to distinguish between cell populations even when volume and mass appear to remain constant. Here we expand up...

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Bibliographic Details
Published in:Lab on a chip 2014-02, Vol.14 (3), p.569-576
Main Authors: Bryan, Andrea K, Hecht, Vivian C, Shen, Wenjiang, Payer, Kristofor, Grover, William H, Manalis, Scott R
Format: Article
Language:English
Subjects:
Animals
Biotechnology
Cancer
Cell Count
Cell Line, Tumor
Cell Size
Channels
Density
Devices
Humans
Measuring instruments
Mice
Microchannels
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Povidone - chemistry
Resonators
Silicon Dioxide - chemistry
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Summary:Cell size, measured as either volume or mass, is a fundamental indicator of cell state. Far more tightly regulated than size is density, the ratio between mass and volume, which can be used to distinguish between cell populations even when volume and mass appear to remain constant. Here we expand upon a previous method for measuring cell density involving a suspended microchannel resonator (SMR). We introduce a new device, the dual SMR, as a high-precision instrument for measuring single-cell mass, volume, and density using two resonators connected by a serpentine fluidic channel. The dual SMR designs considered herein demonstrate the critical role of channel geometry in ensuring proper mixing and damping of pressure fluctuations in microfluidic systems designed for precision measurement. We use the dual SMR to compare the physical properties of two well-known cancer cell lines: human lung cancer cell H1650 and mouse lymphoblastic leukemia cell line L1210.
ISSN:1473-0197
1473-0189
DOI:10.1039/c3lc51022k