NMDA receptor activation requires remodeling of inter-subunit contacts within ligand-binding heterodimers

• Published in: Nature communications 2011-10, Vol.2, p.498-498
• Main Authors: Borschel, William F, Murthy, Swetha E, Kasperek, Eileen M, Popescu, Gabriela K
• Format: Article
• Language: English
• ISSN: 2041-1723
• DOI: 10.1038/ncomms1512

Two classes of glutamate-activated channels mediate excitation at central synapses: N -methyl- d -aspartic acid (NMDA) receptors and non-NMDA receptors. Despite substantial structural homology, each class generates signals with characteristic kinetics and mediates distinct synaptic functions. In non-NMDA receptors, the strength of inter-subunit contacts within agonist-binding domains is inversely correlated with functional desensitization. Here we test how the strength of these contacts affects NMDA receptor activation by combining mutagenesis and single-channel current analyses. We show that receptors with covalently linked dimers had dramatically lower activity due to high barriers to opening and unstable open states but had intact desensitization. Based on these observations, we suggest that in NMDA receptors rearrangements at the heterodimer interface represent an early and integral step of the opening sequence but are not required for desensitization. These results demonstrate distinct functional roles in the activation of NMDA and non-NMDA glutamate-gated channels for largely conserved inter-subunit contacts.