Applied osmotic loading for promoting development of engineered cartilage

Abstract This study investigated the potential use of static osmotic loading as a cartilage tissue engineering strategy for growing clinically relevant grafts from either synovium-derived stem cells (SDSCs) or chondrocytes. Bovine SDSCs and chondrocytes were individually encapsulated in 2% w/v agaro...

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Bibliographic Details
Published in:Journal of biomechanics 2013-10, Vol.46 (15), p.2674-2681
Main Authors: Sampat, Sonal R, Dermksian, Matthew V, Oungoulian, Sevan R, Winchester, Robert J, Bulinski, J. Chloë, Ateshian, Gerard A, Hung, Clark T
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Arthritis
Cartilage
Cartilage - cytology
Cartilage - metabolism
Cattle
Cells, Cultured
Chondrocytes
Chondrocytes - cytology
Chondrocytes - metabolism
Collagen
Construction
Elastic Modulus
Encapsulation
Media
Modulus of elasticity
Osmotic Pressure - physiology
Physical Medicine and Rehabilitation
Static osmotic loading
Stem cells
Strategy
Synovium-derived stem cells
Time Factors
Tissue Engineering
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Summary:Abstract This study investigated the potential use of static osmotic loading as a cartilage tissue engineering strategy for growing clinically relevant grafts from either synovium-derived stem cells (SDSCs) or chondrocytes. Bovine SDSCs and chondrocytes were individually encapsulated in 2% w/v agarose and divided into chondrogenic media of osmolarities 300 (hypotonic), 330 (isotonic), and 400 (hypertonic, physiologic) mOsM for up to 7 weeks. The application of hypertonic media to constructs comprised of SDSCs or chondrocytes led to increased mechanical properties as compared to hypotonic (300 mOsM) or isotonic (330 mOsM) media ( p
ISSN:0021-9290
1873-2380
DOI:10.1016/j.jbiomech.2013.07.043