UV-C response of the ribonucleotide reductase large subunit involves both E2F-mediated gene transcriptional regulation and protein subcellular relocalization in tobacco cells

E2F factors are implicated in various cellular processes including specific gene induction at the G1/S transition of the cell cycle. We present in this study a novel regulatory aspect for the tobacco large subunit of ribonucleotide reductase (R1a) and its encoding gene (RNR1a) in the UV‐C response....

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Bibliographic Details
Published in:Nucleic acids research 2004, Vol.32 (4), p.1430-1438
Main Authors: Lincker, F, Philipps, G, Chaboute, M.E
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
Base Sequence
Binding Sites
Cell Cycle Proteins
Cell Line
cell nucleus
Cell Nucleus - enzymology
cell suspension culture
DNA Replication
DNA-Binding Proteins - physiology
E2F Transcription Factors
gene expression regulation
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Molecular Sequence Data
Nicotiana
Nicotiana - cytology
Nicotiana - enzymology
Nicotiana - genetics
Nicotiana - radiation effects
Nicotiana tabacum
promoter regions
Promoter Regions, Genetic
Protein Subunits - analysis
Protein Subunits - genetics
Protein Subunits - metabolism
protein transport
Response Elements
ribonucleotide reductase
Ribonucleotide Reductases - analysis
Ribonucleotide Reductases - genetics
Ribonucleotide Reductases - metabolism
RNR1a gene
S Phase
structural genes
tobacco
transcription (genetics)
transcription factors
Transcription Factors - physiology
Transcriptional Activation
ultraviolet radiation
Ultraviolet Rays
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Summary:E2F factors are implicated in various cellular processes including specific gene induction at the G1/S transition of the cell cycle. We present in this study a novel regulatory aspect for the tobacco large subunit of ribonucleotide reductase (R1a) and its encoding gene (RNR1a) in the UV‐C response. By structural analyses, two E2F sites were identified on the promoter of this gene. Functional analysis showed that, in addition to their role in the specific G1/S induction of the RNR1a gene, both E2F sites were important for regulating specific RNR1a gene expression in response to UV‐C irradiation in non‐synchronized and synchronized cells. Concomitantly, western blot and cellular analyses showed an increase of a 60 kDa E2F factor and a transient translocation of a GFP‐R1a protein fusion from cytoplasm to nucleus in response to UV irradiation.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkh310