Bacterial diversity associated with the tunic of the model chordate Ciona intestinalis

The sea squirt Ciona intestinalis is a well-studied model organism in developmental biology, yet little is known about its associated bacterial community. In this study, a combination of 454 pyrosequencing of 16S ribosomal RNA genes, catalyzed reporter deposition-fluorescence in situ hybridization a...

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Bibliographic Details
Published in:The ISME Journal 2014-02, Vol.8 (2), p.309-320
Main Authors: Blasiak, Leah C, Zinder, Stephen H, Buckley, Daniel H, Hill, Russell T
Format: Article
Language:English
Subjects:
631/326/2565/2134
631/326/41/547
Animals
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - growth & development
Bacterial Physiological Phenomena
Biodiversity
Biomedical and Life Sciences
Chemical analysis
Chordata
Ciona intestinalis
Ciona intestinalis - microbiology
Community structure
Ecology
Evolutionary Biology
Flavobacteria
Fluorescence
Hybridization
In Situ Hybridization, Fluorescence
Life Sciences
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Molecular Sequence Data
Original
original-article
Phylogeny
RNA, Ribosomal, 16S - genetics
Seawater
Seawater - microbiology
Water analysis
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Summary:The sea squirt Ciona intestinalis is a well-studied model organism in developmental biology, yet little is known about its associated bacterial community. In this study, a combination of 454 pyrosequencing of 16S ribosomal RNA genes, catalyzed reporter deposition-fluorescence in situ hybridization and bacterial culture were used to characterize the bacteria living inside and on the exterior coating, or tunic, of C. intestinalis adults. The 454 sequencing data set demonstrated that the tunic bacterial community structure is different from that of the surrounding seawater. The observed tunic bacterial consortium contained a shared community of
ISSN:1751-7362
1751-7370
DOI:10.1038/ismej.2013.156