General Method for Regulating Protein Stability with Light

Post-translational regulation of protein abundance in cells is a powerful tool for studying protein function. Here, we describe a novel genetically encoded protein domain that is degraded upon exposure to nontoxic blue light. We demonstrate that fusion proteins containing this domain are rapidly deg...

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Bibliographic Details
Published in:ACS chemical biology 2014-01, Vol.9 (1), p.111-115
Main Authors: Bonger, Kimberly M, Rakhit, Rishi, Payumo, Alexander Y, Chen, James K, Wandless, Thomas J
Format: Article
Language:English
Subjects:
Animals
Avena - chemistry
Avena - genetics
Light
Luminescent Proteins - chemistry
Luminescent Proteins - genetics
Mice
NIH 3T3 Cells
Photolysis
Phototropins - chemistry
Phototropins - genetics
Protein Engineering
Protein Stability
Protein Structure, Tertiary
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - genetics
Zebrafish
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Description
Summary:Post-translational regulation of protein abundance in cells is a powerful tool for studying protein function. Here, we describe a novel genetically encoded protein domain that is degraded upon exposure to nontoxic blue light. We demonstrate that fusion proteins containing this domain are rapidly degraded in cultured cells and in zebrafish upon illumination.
ISSN:1554-8929
1554-8937
DOI:10.1021/cb400755b