Modification and Secretion of Human Interleukin 2 Produced in Insect Cells by a Baculovirus Expression Vector

A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr15,500 IL-2 polypeptide, the majority of which was secreted into the cul...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-12, Vol.82 (24), p.8404-8408
Main Authors: Smith, Gale E., Ju, Grace, Ericson, Brad L., Moschera, John, Lahm, Hans-Werner, Chizzonite, Richard, Summers, Max D.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Biological and medical sciences
Biotechnology
Cell lines
Fundamental and applied biological sciences. Psychology
Gels
Gene Expression Regulation
Genetic engineering
Genetic technics
Genetic Vectors
Glycoproteins - genetics
Glycoproteins - metabolism
Humans
Insect genetics
Insect vectors
Insect Viruses - genetics
Insecta - genetics
Interleukin-2 - biosynthesis
Interleukin-2 - genetics
Interleukin-2 - metabolism
Messenger RNA
Methods. Procedures. Technologies
Molecular cloning
Molecular Weight
Protein Processing, Post-Translational
Proteins
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
T lymphocytes
Viruses
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Description
Summary:A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr15,500 IL-2 polypeptide, the majority of which was secreted into the culture medium during infection. The recombinant IL-2 was able to stimulate the growth of an IL-2-dependent cell line. The N-terminal amino acid sequence of the insect-derived IL-2 was identical to that of natural IL-2. Thus, a mammalian signal peptide was recognized and properly removed in insect cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.24.8404