Chymase activities and survival in endotoxin-induced human chymase transgenic mice

We examined the effects of overexpressed human chymase on survival and activity in lipopolysaccharide (LPS)-treated mice. Human chymase transgenic (Tg) and wild-type C57BL/6 (WT) mice were treated with LPS (0.03, 0.1 and 0.3 mg/day; intraperitoneal) for 2 weeks. Treatment with 0.03 mg LPS did not af...

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Bibliographic Details
Published in:International journal of medical sciences 2014-01, Vol.11 (3), p.222-225
Main Authors: Rafiq, Kazi, Fan, Yu-Yan, Sherajee, Shamshad J, Takahashi, Yoshimasa, Matsuura, Junji, Hase, Naoki, Mori, Hirohito, Nakano, Daisuke, Kobara, Hideki, Hitomi, Hirofumi, Masaki, Tsutomu, Urata, Hidenori, Nishiyama, Akira
Format: Article
Language:English
Subjects:
Animals
Chymases - biosynthesis
Chymases - genetics
Gene Expression Regulation, Enzymologic - drug effects
Humans
Lipopolysaccharides - toxicity
Mice, Transgenic
Myocardium - enzymology
Short Research Communication
Skin - drug effects
Skin - enzymology
Survival
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Summary:We examined the effects of overexpressed human chymase on survival and activity in lipopolysaccharide (LPS)-treated mice. Human chymase transgenic (Tg) and wild-type C57BL/6 (WT) mice were treated with LPS (0.03, 0.1 and 0.3 mg/day; intraperitoneal) for 2 weeks. Treatment with 0.03 mg LPS did not affect survival in either WT or Tg mice. WT mice were not affected by 0.1 mg/day of LPS, whereas 25% of Tg mice died. Survival of mice treated with 0.3 mg/day of LPS was 87.5% and 0% in WT and Tg, respectively. LPS-induced increases in chymase activity in the heart and skin were significantly greater in Tg than WT mice. These data suggest a possible contribution of human chymase activation to LPS-induced mortality.
ISSN:1449-1907
1449-1907
DOI:10.7150/ijms.7382