Optical axial scanning in confocal microscopy using an electrically tunable lens

This paper presents the use and characterization of an electrically focus tunable lens to perform axial scanning in a confocal microscope. Lateral and axial resolution are characterized over a >250 µm axial scan range. Confocal microscopy using optical axial scanning is demonstrated in epithelial...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical optics express 2014-02, Vol.5 (2), p.645-652
Main Authors: Jabbour, Joey M, Malik, Bilal H, Olsovsky, Cory, Cuenca, Rodrigo, Cheng, Shuna, Jo, Javier A, Cheng, Yi-Shing Lisa, Wright, John M, Maitland, Kristen C
Format: Article
Language:English
Subjects:
Microscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This paper presents the use and characterization of an electrically focus tunable lens to perform axial scanning in a confocal microscope. Lateral and axial resolution are characterized over a >250 µm axial scan range. Confocal microscopy using optical axial scanning is demonstrated in epithelial tissue and compared to traditional stage scanning. By enabling rapid axial scanning, minimizing motion artifacts, and reducing mechanical complexity, this technique has potential to enhance in vivo three-dimensional imaging in confocal endomicroscopy.
ISSN:2156-7085
2156-7085
DOI:10.1364/boe.5.000645