Recovery of neurological function of ischemic stroke by application of conditioned medium of bone marrow mesenchymal stem cells derived from normal and cerebral ischemia rats

• Published in: Journal of biomedical science 2014-01, Vol.21 (1), p.5-5, Article 5
• Main Authors: Tsai, May-Jywan, Tsai, Shen-Kou, Hu, Bo-Ruei, Liou, Dann-Ying, Huang, Shih-Ling, Huang, Ming-Chao, Huang, Wen-Cheng, Cheng, Henrich, Huang, Shiang-Suo
• Format: Article
• Language: English
• Subjects: Animals; Bone marrow; Bone Marrow Cells - cytology; Brain; Brain Ischemia - genetics; Brain Ischemia - physiopathology; Brain Ischemia - therapy; Cell- and Tissue-Based Therapy; Cerebral ischemia; Culture Media, Conditioned - pharmacology; Health aspects; Humans; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells - cytology; Neurons; Rats; Rodents; Stem cells; Stroke; Stroke - physiopathology; Stroke - therapy
• ISSN: 1423-0127; 1021-7770; 1423-0127
• DOI: 10.1186/1423-0127-21-5

Several lines of evidence have demonstrated that bone marrow-derived mesenchymal stem cells (BM-MSC) release bioactive factors and provide neuroprotection for CNS injury. However, it remains elusive whether BM-MSC derived from healthy donors or stroke patients provides equal therapeutic potential. The present work aims to characterize BM-MSC prepared from normal healthy rats (NormBM-MSC) and cerebral ischemia rats (IschBM-MSC), and examine the effects of their conditioned medium (Cm) on ischemic stroke animal model. Isolated NormBM-MSC or IschBM-MSC formed fibroblastic like morphology and expressed CD29, CD90 and CD44 but failed to express the hematopoietic marker CD34. The number of colony formation of BM-MSC was more abundant in IschBM-MSC than in NormBM-MSC. This is in contrast to the amount of Ficoll-fractionated mononuclear cells from normal donor and ischemic rats. The effect of cm of BM-MSC was further examined in cultures and in middle cerebral artery occlusion (MCAo) animal model. Both NormBM-MSC Cm and IschBM-MSC Cm effectively increased neuronal connection and survival in mixed neuron-glial cultures. In vivo, intravenous infusion of NormBM-MSC Cm and IschBM-MSC Cm after stroke onset remarkably improved functional recovery. Furthermore, NormBM-MSC Cm and IschBM-MSC Cm increased neurogenesis and attenuated microglia/ macrophage infiltration in MCAo rat brains. Our data suggest equal effectiveness of BM-MSC Cm derived from ischemic animals or from a normal population. Our results thus revealed the potential of BM-MSC Cm on treatment of ischemic stroke.