Modulation of c-Fos and BDNF Protein Expression in Pentylenetetrazole-Kindled Mice following the Treatment with Novel Antiepileptic Compound HHL-6

Brain-derived neurotrophic factor (BDNF) and c-Fos are shown to promote epileptogenesis and are taken as a marker of neuronal activity. The present study investigated the expression of BDNF and c-Fos in mice brain with pentylenetetrazol- (PTZ-) induced generalized seizure and evaluated the effect of...

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-9
Main Authors: Malhi, Saima Mahmood, Jawed, Huma, Hanif, Farina, Ashraf, Nadeem, Zubair, Farhat, Siddiqui, Bina S., Begum, Sabira, Kabir, Nurul, Simjee, Shabana Usman
Format: Article
Language:English
Subjects:
Animals
Anticonvulsants
Anticonvulsants - administration & dosage
Behavior
Biomedical research
Brain - drug effects
Brain - metabolism
Brain - pathology
Brain research
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor - biosynthesis
Brain-Derived Neurotrophic Factor - genetics
Disease Models, Animal
Dosage and administration
Drug dosages
Drug interactions
Epilepsy
Epilepsy - drug therapy
Epilepsy - genetics
Gene expression
Gene Expression Regulation - drug effects
Genes, fos - genetics
Health aspects
Kinases
Laboratory animals
Mice
Patient outcomes
Pentylenetetrazole - administration & dosage
Pharmacology, Experimental
Proteins
Proto-oncogenes
Rodents
Seizures - drug therapy
Seizures - pathology
Studies
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Summary:Brain-derived neurotrophic factor (BDNF) and c-Fos are shown to promote epileptogenesis and are taken as a marker of neuronal activity. The present study investigated the expression of BDNF and c-Fos in mice brain with pentylenetetrazol- (PTZ-) induced generalized seizure and evaluated the effect of novel tryptamine derivative HHL-6 on the expression of these two markers. The subconvulsive dose of PTZ (50 mg/kg) was administered on alternate days in the experimental groups until the seizure scores 4-5 developed in the PTZ-control group. At the end of each experiment, animals were sacrificed, brain samples were collected and cryosectioned, and immunohistochemical analysis of BDNF and c-Fos protein was performed. Data obtained from two sections per mouse (n=12 animals/group) is presented as means ± S.E.M. The test compound HHL-6 demonstrated a potent anticonvulsant activity in the PTZ-induced seizure in mice. Significant reduction in the BDNF (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/876712