First report on isolation and characterization of leishmania major from meriones hurrianae (rodentia : gerbillidae) of a rural cutaneous leishmaniasis focus in South-Eastern Iran

• Published in: Iranian red crescent medical journal 2013-09, Vol.15 (9), p.789-793
• Main Authors: Kassiri, Hamid, Javadian, Izat al-Din, Naddaf, Rida Said, Mohebali, Mahdi
• Format: Article
• Language: English
• Subjects: leishmaniasis; داء الليشمانيات
• ISSN: 2074-1804; 2074-1812
• DOI: 10.5812/ircmj.6974

Background : Zoonotic Cutaneous Leishmaniasis (ZCL) is an endemic health problem in many rural areas of Iran, with doubled number of incidences over the last decade. Different species of rodents serve as natural reservoir host for ZCL. The disease is considered as a major health problem in rural areas of Mirjaveh, Chabahar, and Konarak Counties of Sistan va Baluchistan Province. Objectives : This study describes the identity of Leishmania species, isolated from Meriones hurrianae from Chabahar County using RAPD-PCR methodology. Materials and Methods : Rodents were entrapped by live traps baited with roasted walnut, tomato, and cucumber during spring and summer. All rodents were identified based on external features including fur color, ears characteristics, tail length, hind feet, body measurements, and internal features of teeth and cranium. Giemsa-stained impressions from rodents’ ears were examined for amastigotes microscopically. The samples from infected rodents were cultured in NNN+LIT medium and then the harvested parasites at the stationary phase were subjected to DNA extraction followed by amplification with RAPD-PCR. Results : All the 28 entrapped animals were identified as M. hurrianae. Five animals showed to harbor Leishmania parasite by microscopy. Leishmania DNA isolated from five M. hurrianae produced distinctive bands of L. major with four primers. However, the products that were amplified with primers AB1-07, 327, and 329 were stable and reproducible. This is the first report on the isolation and identification of L. major from M. hurrianae from Iran. Conclusions : Regarding infection rate of 17.8 %, M. hurrianae seems to play the major role in the maintenance and transmission of disease to humans in this area.