Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

Surgical instruments are often strongly contaminated with patients’ blood and tissues, possibly containing pathogens. The reuse of contaminated instruments without adequate cleaning and sterilization can cause postoperative inflammation and the transmission of infectious diseases from one patient to...

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-8
Main Authors: Wilhelm, Nadja, Perle, Nadja, Simmoteit, Robert, Schlensak, Christian, Wendel, Hans-Peter, Avci-Adali, Meltem
Format: Article
Language:English
Subjects:
Animals
Centrifugation
Creutzfeldt-Jakob disease
Deoxyribonucleic acid
DNA
DNA Primers - metabolism
DNA, Single-Stranded - isolation & purification
Efficiency
Genome - genetics
Humans
Limit of Detection
Medical equipment
Methods
Molecular weight
Oligonucleotides
Oligonucleotides - isolation & purification
Physiological apparatus
Proteins
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Sepsis
Sterilization - methods
Studies
Surgical apparatus & instruments
Surgical Instruments
Urea
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Summary:Surgical instruments are often strongly contaminated with patients’ blood and tissues, possibly containing pathogens. The reuse of contaminated instruments without adequate cleaning and sterilization can cause postoperative inflammation and the transmission of infectious diseases from one patient to another. Thus, based on the stringent sterility requirements, the development of highly efficient, validated cleaning processes is necessary. Here, we use for the first time synthetic single-stranded DNA (ssDNA_ODN), which does not appear in nature, as a test soiling to evaluate the cleaning efficiency of routine washing processes. Stainless steel test objects were coated with a certain amount of ssDNA_ODN. After cleaning, the amount of residual ssDNA_ODN on the test objects was determined using quantitative real-time PCR. The established method is highly specific and sensitive, with a detection limit of 20 fg, and enables the determination of the cleaning efficiency of medical cleaning processes under different conditions to obtain optimal settings for the effective cleaning and sterilization of instruments. The use of this highly sensitive method for the validation of cleaning processes can prevent, to a significant extent, the insufficient cleaning of surgical instruments and thus the transmission of pathogens to patients.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/632127