Purine biosynthesis-deficient Burkholderia mutants are incapable of symbiotic accommodation in the stinkbug

• Published in: The ISME Journal 2014-03, Vol.8 (3), p.552-563
• Main Authors: Kim, Jiyeun Kate, Jang, Ho Am, Won, Yeo Jin, Kikuchi, Yoshitomo, Heum Han, Sang, Kim, Chan-Hee, Nikoh, Naruo, Fukatsu, Takema, Lee, Bok Luel
• Format: Article
• Language: English
• Subjects: 631/326/2565/855; 631/326/41/547; Animals; Biofilms; Biomedical and Life Sciences; Biosynthesis; Burkholderia; Burkholderia - genetics; Burkholderia - growth & development; Burkholderia - physiology; Ecology; Evolutionary Biology; Genes, Insect; Heteroptera - growth & development; Heteroptera - microbiology; Heteroptera - physiology; Insects; Life Sciences; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Molecular Sequence Data; Mutation; Original; original-article; Purines - biosynthesis; Symbiosis
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/ismej.2013.168

The Riptortus–Burkholderia symbiotic system represents a promising experimental model to study the molecular mechanisms involved in insect–bacterium symbiosis due to the availability of genetically manipulated Burkholderia symbiont. Using transposon mutagenesis screening, we found a symbiosis-deficient mutant that was able to colonize the host insect but failed to induce normal development of host’s symbiotic organ. The disrupted gene was identified as purL involved in purine biosynthesis. In vitro growth impairment of the purL mutant and its growth dependency on adenine and adenosine confirmed the functional disruption of the purine synthesis gene. The purL mutant also showed defects in biofilm formation, and this defect was not rescued by supplementation of purine derivatives. When inoculated to host insects, the purL mutant was initially able to colonize the symbiotic organ but failed to attain a normal infection density. The low level of infection density of the purL mutant attenuated the development of the host’s symbiotic organ at early instar stages and reduced the host’s fitness throughout the nymphal stages. Another symbiont mutant-deficient in a purine biosynthesis gene, purM , showed phenotypes similar to those of the purL mutant both in vitro and in vivo , confirming that the purL phenotypes are due to disrupted purine biosynthesis. These results demonstrate that the purine biosynthesis genes of the Burkholderia symbiont are critical for the successful accommodation of symbiont within the host, thereby facilitating the development of the host’s symbiotic organ and enhancing the host’s fitness values.