17β‐Estradiol Protects Human Eyelid‐Derived Adipose Stem Cells against Cytotoxicity and Increases Transplanted Cell Survival in Spinal Cord injury

Stem cell transplantation represents a promising strategy for the repair of spinal cord injury (SCI). However, the low survival rate of the grafted cells is a major obstacle hindering clinical success because of ongoing secondary injury processes, which includes excitotoxicity, inflammation and oxid...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular and molecular medicine 2014-02, Vol.18 (2), p.326-343
Main Authors: Zhou, Jing, Lu, Ping, Ren, Hao, Zheng, Zefeng, Ji, Junfeng, Liu, Hua, Jiang, Fangzhen, Ling, Shucai, Heng, Boon Chin, Hu, Xueqing, Ouyang, HongWei
Format: Article
Language:English
Subjects:
17β-Estradiol
17‐β‐estradiol
Adipose Tissue - cytology
Adipose Tissue - immunology
Animals
Apoptosis
Apoptosis - drug effects
Cell adhesion & migration
Cell death
Cell survival
cell transplantation
Cytotoxicity
Estradiol - pharmacology
Excitotoxicity
Eyelid
Eyelids - cytology
Eyelids - immunology
Gene expression
Graft Survival - drug effects
Graft Survival - immunology
Growth factors
hEASCs
Humans
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Immunoglobulins
Inflammation
Intercellular Signaling Peptides and Proteins - biosynthesis
Intercellular Signaling Peptides and Proteins - secretion
Male
Original
Oxidative stress
Penicillin
Proteins
Rats
Rats, Sprague-Dawley
Recovery of Function
Regeneration
Secretion
Spinal cord injuries
Spinal Cord Injuries - immunology
Spinal Cord Injuries - pathology
Spinal Cord Injuries - therapy
spinal cord injury
Stem Cell Transplantation
Stem cells
Stem Cells - cytology
Stem Cells - drug effects
Stem Cells - immunology
Survival
Transplantation
Transplantation, Heterologous
Treatment Outcome
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Stem cell transplantation represents a promising strategy for the repair of spinal cord injury (SCI). However, the low survival rate of the grafted cells is a major obstacle hindering clinical success because of ongoing secondary injury processes, which includes excitotoxicity, inflammation and oxidative stress. Previous studies have shown that 17b‐estradiol (E2) protects several cell types against cytotoxicity. Thus, we examined the effects of E2 on the viability of human eyelid adipose‐derived stem cells (hEASCs) in vitro with hydrogen peroxide (H2O2)‐induced cell model and in vivo within a rat SCI model. Our results showed that E2 protected hEASCs against H2O2‐induced cell death in vitro, and enhanced the survival of grafted hEASCs in vivo by reducing apoptosis. Additionally, E2 also enhanced the secretion of growth factors by hEASCs, thereby making the local microenvironment more conducive for tissue regeneration. Overall, E2 administration enhanced the therapeutic efficacy of hEASCs transplantation and facilitated motor function recovery after SCI. Hence, E2 administration may be an intervention of choice for enhancing survival of transplanted hEASCs after SCI.
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.12191