Application of higher derivative techniques to analysis of high-resolution thermal denaturation profiles of reassociated repetitive DNA [Mung beans, peas]

We have analyzed high-resolution denaturation profiles of reassociated repetitive DNA sequences by using a combination of higher derivative analysis and curve-fitting techniques. Procedures originally used for resolution of components in complex absorption spectra were found to be applicable to high...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1978-12, Vol.75 (12), p.6026-6030
Main Authors: Cuellar, Richard E., Ford, Glenn A., Briggs, Winslow R., Thompson, William F.
Format: Article
Language:English
Subjects:
Base pair mismatch
Base Sequence
Computers
Cots
Curves
Digitization
DNA
DNA - genetics
DNA, Bacterial - genetics
Escherichia coli
Hot Temperature
Hydrogen Bonding
Melting
Nucleic Acid Denaturation
Nucleotide sequences
Peas
Plants
Repetitive sequences
Tetraethylammonium Compounds - pharmacology
Thermal stability
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Summary:We have analyzed high-resolution denaturation profiles of reassociated repetitive DNA sequences by using a combination of higher derivative analysis and curve-fitting techniques. Procedures originally used for resolution of components in complex absorption spectra were found to be applicable to high-resolution analysis of melting profiles of reassociated repetitive DNA sequences from pea DNA. Under conditions that eliminate the base composition effect on thermal stability (2.4 M tetraethylammonium chloride), such an analysis can distinguish ``thermal classes'' of repetitive DNA duplexes exhibiting different amounts of base pair mismatch. Only a single thermal class is observed in reassociated Escherichia coli DNA whereas at least five classes can be reproducibly distinguished in pea and mung bean DNAs.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.75.12.6026