Label-Free Quantitative Imaging of Cholesterol in Intact Tissues by Hyperspectral Stimulated Raman Scattering Microscopy

A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label‐free imag...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2013-12, Vol.52 (49), p.13042-13046
Main Authors: Wang, Ping, Li, Junjie, Wang, Pu, Hu, Chun-Rui, Zhang, Delong, Sturek, Michael, Cheng, Ji-Xin
Format: Article
Language:English
Subjects:
Arteries - chemistry
Atherosclerosis - pathology
cholesterol
Cholesterol - analysis
Cholesterol - chemistry
Humans
lipids
Spectrum Analysis, Raman - methods
stimulated Raman scattering
tissue analysis
Tissue Array Analysis - methods
vibrational spectroscopy
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Summary:A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label‐free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201306234