Isolation of a Developmental Gene of Bacillus subtilis and Its Expression in Escherichia coli

Glucose dehydrogenase of Bacillus subtilis is a developmental enzyme that is not found in growing (vegetative) cells but is synthesized after the differentiation process that leads to the production of endospores has started. We have isolated the gene coding for this enzyme from a λ Charon 4A phage...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1983-02, Vol.80 (3), p.785-789
Main Authors: Vasantha, N., Uratani, Brenda, Ramaley, Robert F., Freese, Ernst
Format: Article
Language:English
Subjects:
Antibodies
Bacillus subtilis - genetics
Bacteriophage lambda
Bacteriophages
Carbohydrate Dehydrogenases - genetics
DNA
Enzymes
Escherichia coli - genetics
Gels
Gene Expression Regulation
Genes
Germination
Glucose Dehydrogenases - genetics
Operon
Plasmids
Somatic cells
Spores, Bacterial
Sporulation
Transcription, Genetic
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Summary:Glucose dehydrogenase of Bacillus subtilis is a developmental enzyme that is not found in growing (vegetative) cells but is synthesized after the differentiation process that leads to the production of endospores has started. We have isolated the gene coding for this enzyme from a λ Charon 4A phage library of B. subtilis DNA. It is transcribed and translated in vegetative cells of the nondifferentiating organism Escherichia coli into enzymatically active glucose dehydrogenase that has the same physicochemical properties as the enzyme produced in B. subtilis during sporulation. Subcloning of the λ DNA insert into pBR322 plasmid derivatives showed that the glucose dehydrogenase gene was transcribed in E. coli from a promoter within the B. subtilis genome.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.3.785