Microperfusion of 3-MPA into the brain augments GABA

Abstract In vivo effects of microperfusion of a GABA synthesis inhibitor (3-MPA) into the striatum and hippocampus on amino acid concentrations and electrical neuronal activity were investigated. Paradoxical elevations in GABA in the striatum (5-fold in anesthetized and 50-fold in awake rats) and hi...

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Bibliographic Details
Published in:Epilepsy & behavior 2013-12, Vol.29 (3), p.478-484
Main Authors: Mayer, Andrew P, Osorio, Ivan, Lunte, Craig E
Format: Article
Language:English
Subjects:
3-Mercaptopropionic acid (3-MPA)
3-Mercaptopropionic Acid - pharmacology
Analytes
Animals
Brain - anatomy & histology
Brain - drug effects
Brain - metabolism
Chromatography, High Pressure Liquid
Convulsants - pharmacology
ECoG
Electrochemical Techniques
Electrodes, Implanted
Electroencephalography
gamma-Aminobutyric Acid - metabolism
Glutamic acid (glutamate)
Male
Microdialysis
Neurology
Neurotransmitter Agents - metabolism
Paradoxical
Rats
Rats, Wistar
Steady-state
Theory
Time Factors
Wakefulness
γ-Aminobutyric acid (GABA)
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Summary:Abstract In vivo effects of microperfusion of a GABA synthesis inhibitor (3-MPA) into the striatum and hippocampus on amino acid concentrations and electrical neuronal activity were investigated. Paradoxical elevations in GABA in the striatum (5-fold in anesthetized and 50-fold in awake rats) and hippocampus (2-fold in anesthetized and 15-fold in awake rats) were documented under steady-state concentrations of 3-MPA along with expected increases in glutamate (a 15-fold increase and a 250-fold increase in the striatum of anesthetized and awake rats, respectively; a 7-fold increase and a 25-fold increase in the hippocampus of anesthetized and awake rats, respectively). There was no clear epileptiform or seizure activity. Explanations for the paradoxical increase in GABA are offered, and emphasis is placed on the dependency of disinhibition on the model in which its effects are studied as well as on the prevailing level of activation of the probed network.
ISSN:1525-5050
1525-5069
DOI:10.1016/j.yebeh.2013.08.026